The defective En-I102 element encodes a product reducing the mutability of the En/Spm transposable element system of Zea mays.

H. Cuypers, S. Dash, P. A. Peterson, H. Saedler, A. Gierl

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

Genetic and molecular analysis has revealed a specific En-element of deletion derivative (En-I102) which reduces En/Spm-induced mutability. In the presence of En-I102 the excision frequency of both the autonomous En-1 element and the inhibitor element Spm-I5719A is reduced and excision occurs later in development. The 3697 bp long En-I102 element is derived from En-1 by an internal deletion of 4590 bp removing nucleotides 1862-6451. The promoter at the left end and sequences required for polyadenylation are retained in En-I102. It is transcribed to yield predominantly a 1.8 kb poly(A) RNA. cDNA analysis of this transcript indicated that it contains the coding capacity for a 386 amino acid polypeptide. This polypeptide shares homology with En/Spm encoded functions and we suggest that it interferes with transposition at the protein level.

Original languageEnglish
Pages (from-to)2953-2960
Number of pages8
JournalEMBO Journal
Volume7
Issue number10
DOIs
StatePublished - Oct 1988
Externally publishedYes

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