The combination of miRNA-196b, LCN2, and TIMP1 is a potential set of circulating biomarkers for screening individuals at risk for familial pancreatic cancer

Detlef K. Bartsch, Norman Gercke, Konstantin Strauch, Ronja Wieboldt, Elvira Matthäi, Vinona Wagner, Susanne Rospleszcz, Agnes Schäfer, Frederike S. Franke, Ioannis Mintziras, Christian Bauer, Tobias Grote, Jens Figiel, Pietro Di Fazio, Andreas Burchert, Silke Reinartz, Elke Pogge von Strandmann, Günter Klöppel, Emily P. Slater

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Individuals at risk (IAR) of familial pancreatic cancer (FPC) are good candidates for screening. Unfortunately, neither reliable imaging modalities nor biomarkers are available to detect high-grade precursor lesions or early cancer. Circulating levels of candidate biomarkers LCN2, TIMP1, Glypican-1, RNU2-1f, and miRNA-196b were analyzed in 218 individuals with sporadic pancreatic ductal adenocarcinoma (PDAC, n = 50), FPC (n = 20), chronic pancreatitis (n = 10), IAR with relevant precursor lesions (n = 11) or non-relevant lesions (n = 5), 20 controls, and IAR with (n = 51) or without (n = 51) lesions on pancreatic imaging. In addition, corresponding duodenal juice samples were analyzed for Glypican-1 (n = 144) enrichment and KRAS mutations (n = 123). The panel miR-196b/LCN2/TIMP1 could distinguish high-grade lesions and stage I PDAC from controls with absolute specificity and sensitivity. In contrast, Glypican-1 enrichment in serum exosomes and duodenal juice was not diagnostic. KRAS mutations in duodenal juice were detected in 9 of 12 patients with PDAC and only 4 of 9 IAR with relevant precursor lesions. IAR with lesions on imaging had elevated miR-196b/LCN2/TIMP1 levels (p = 0.0007) and KRAS mutations in duodenal juice (p = 0.0004) significantly more often than IAR without imaging lesions. The combination miR-196b/LCN2/TIMP1 might be a promising biomarker set for the detection of high-grade PDAC precursor lesions in IAR of FPC families.

Original languageEnglish
Article number295
JournalJournal of Clinical Medicine
Volume7
Issue number10
DOIs
StatePublished - Oct 2018

Keywords

  • Glypican-1
  • KRAS mutation
  • LCN2/NGAL
  • Pancreatic ductal adenocarcinoma
  • TIMP1
  • miRNA-196b

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