The Arabidopsis NPF3 protein is a GA transporter

Iris Tal, Yi Zhang, Morten Egevang Jørgensen, Odelia Pisanty, Inês C.R. Barbosa, Melina Zourelidou, Thomas Regnault, Christoph Crocoll, Carl Erik Olsen, Roy Weinstain, Claus Schwechheimer, Barbara Ann Halkier, Hussam Hassan Nour-Eldin, Mark Estelle, Eilon Shani

Research output: Contribution to journalArticlepeer-review

160 Scopus citations

Abstract

Gibberellins (GAs) are plant hormones that promote a wide range of developmental processes. While GA signalling is well understood, little is known about how GA is transported or how GA distribution is regulated. Here we utilize fluorescently labelled GAs (GA-Fl) to screen for Arabidopsis mutants deficient in GA transport. We show that the NPF3 transporter efficiently transports GA across cell membranes in vitro and GA-Fl in vivo. NPF3 is expressed in root endodermis and repressed by GA. NPF3 is targeted to the plasma membrane and subject to rapid BFA-dependent recycling. We show that abscisic acid (ABA), an antagonist of GA, is also transported by NPF3 in vitro. ABA promotes NPF3 expression and GA-Fl uptake in plants. On the basis of these results, we propose that GA distribution and activity in Arabidopsis is partly regulated by NPF3 acting as an influx carrier and that GA-ABA interaction may occur at the level of transport.

Original languageEnglish
Article number11486
JournalNature Communications
Volume7
DOIs
StatePublished - 3 May 2016

Fingerprint

Dive into the research topics of 'The Arabidopsis NPF3 protein is a GA transporter'. Together they form a unique fingerprint.

Cite this