The application of optodes in FIA-based fermentation process control using the software package FIACRE

M. Busch; F. Gutberlet; W. Höbel; J. Polster; H. L. Schmidt; M. Schwenk

doi:10.1016/0925-4005(93)85280-N

The application of optodes in FIA-based fermentation process control using the software package FIACRE

M. Busch, F. Gutberlet, W. Höbel, J. Polster, H. L. Schmidt, M. Schwenk

Life Sciences

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12 Scopus citations

Abstract

Optical biosensors for the determination of substrates like ethanol and urea that can be principally used in fermentation process control are described. The ethanol sensor measures the increase of NADH fluorescence during the enzymatic conversion of ethanol using an enyzme system of ADH and AlDH. The urea sensor is based on a pH sensor measuring the change in fluorescnece of the pH indicator fluorescein. Both sensors are suitable for controlling fermentation processes when ethanol and urea are produced or consumed. For this purpose the software package FIACRE has been developed to run three different FIA processes simultaneously by the use of optical biosensors; it is applied to a model fermentation with Enterobacteriaceae Proteus vulgaris controlled by an urea optode. A decrease of urea during two fermentations over 9 and 24 hours, respectively, is shown.

Original language	English
Pages (from-to)	407-412
Number of pages	6
Journal	Sensors and Actuators, B: Chemical
Volume	11
Issue number	1-3
DOIs	https://doi.org/10.1016/0925-4005(93)85280-N
State	Published - 1 Mar 1993

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title = "The application of optodes in FIA-based fermentation process control using the software package FIACRE",

abstract = "Optical biosensors for the determination of substrates like ethanol and urea that can be principally used in fermentation process control are described. The ethanol sensor measures the increase of NADH fluorescence during the enzymatic conversion of ethanol using an enyzme system of ADH and AlDH. The urea sensor is based on a pH sensor measuring the change in fluorescnece of the pH indicator fluorescein. Both sensors are suitable for controlling fermentation processes when ethanol and urea are produced or consumed. For this purpose the software package FIACRE has been developed to run three different FIA processes simultaneously by the use of optical biosensors; it is applied to a model fermentation with Enterobacteriaceae Proteus vulgaris controlled by an urea optode. A decrease of urea during two fermentations over 9 and 24 hours, respectively, is shown.",

author = "M. Busch and F. Gutberlet and W. H{\"o}bel and J. Polster and Schmidt, {H. L.} and M. Schwenk",

note = "Funding Information: We thank Professor Dr K. Schiigerl of the University of Hannover (Institut fur Tech&he Chemie, Hannover, Germany) for interesting discussions concerning some problems of fermentation monitoring. We thank Mrs A. Papperger for her helpful technical assistance in recording the reflection spectra. Dr M. Liissner (Siid-deutsche Versuchs-und Forschungsanstalt fIir Milch-wirtschaft; Bakteriologisches Institut der Tech&hen Universitiit Mtinchen in Freising-Weihenstephan) is gratefully acknowledged for providing a culture of Pro-tear vulgaris and for useful discussions. The project was supported by the Deutsche Forschungsgemeinschaft (SFB 145, Biokonversion).",

year = "1993",

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doi = "10.1016/0925-4005(93)85280-N",

language = "English",

volume = "11",

pages = "407--412",

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T1 - The application of optodes in FIA-based fermentation process control using the software package FIACRE

AU - Busch, M.

AU - Gutberlet, F.

AU - Höbel, W.

AU - Polster, J.

AU - Schmidt, H. L.

AU - Schwenk, M.

N1 - Funding Information: We thank Professor Dr K. Schiigerl of the University of Hannover (Institut fur Tech&he Chemie, Hannover, Germany) for interesting discussions concerning some problems of fermentation monitoring. We thank Mrs A. Papperger for her helpful technical assistance in recording the reflection spectra. Dr M. Liissner (Siid-deutsche Versuchs-und Forschungsanstalt fIir Milch-wirtschaft; Bakteriologisches Institut der Tech&hen Universitiit Mtinchen in Freising-Weihenstephan) is gratefully acknowledged for providing a culture of Pro-tear vulgaris and for useful discussions. The project was supported by the Deutsche Forschungsgemeinschaft (SFB 145, Biokonversion).

PY - 1993/3/1

Y1 - 1993/3/1

N2 - Optical biosensors for the determination of substrates like ethanol and urea that can be principally used in fermentation process control are described. The ethanol sensor measures the increase of NADH fluorescence during the enzymatic conversion of ethanol using an enyzme system of ADH and AlDH. The urea sensor is based on a pH sensor measuring the change in fluorescnece of the pH indicator fluorescein. Both sensors are suitable for controlling fermentation processes when ethanol and urea are produced or consumed. For this purpose the software package FIACRE has been developed to run three different FIA processes simultaneously by the use of optical biosensors; it is applied to a model fermentation with Enterobacteriaceae Proteus vulgaris controlled by an urea optode. A decrease of urea during two fermentations over 9 and 24 hours, respectively, is shown.

AB - Optical biosensors for the determination of substrates like ethanol and urea that can be principally used in fermentation process control are described. The ethanol sensor measures the increase of NADH fluorescence during the enzymatic conversion of ethanol using an enyzme system of ADH and AlDH. The urea sensor is based on a pH sensor measuring the change in fluorescnece of the pH indicator fluorescein. Both sensors are suitable for controlling fermentation processes when ethanol and urea are produced or consumed. For this purpose the software package FIACRE has been developed to run three different FIA processes simultaneously by the use of optical biosensors; it is applied to a model fermentation with Enterobacteriaceae Proteus vulgaris controlled by an urea optode. A decrease of urea during two fermentations over 9 and 24 hours, respectively, is shown.

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The application of optodes in FIA-based fermentation process control using the software package FIACRE

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