The AFMas a tool for chromosomal dissection - The influence of physical parameters

R. W. Stark; S. Thalhammer; J. Wienberg; W. M. Heckl

doi:10.1007/s003390051205

The AFMas a tool for chromosomal dissection - The influence of physical parameters

R. W. Stark, S. Thalhammer, J. Wienberg, W. M. Heckl

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70 Scopus citations

Abstract

Human metaphase chromosomes were dissected using an atomic force microscope (AFM) in ambient conditions and in buffer. Cutting with z-modulation in air yielded precise cuts at loading forces F> 17 μN with a full width at maximum depth of 90 nm. After dissection, the chromosomal material adhered to the tip to be used for further biochemical processing. In liquids, we measured the effects of different types of buffer solution on swelling of the chromosomes and their elastic behaviour.

Original language	English
Pages (from-to)	S579-S584
Journal	Applied Physics A: Materials Science and Processing
Volume	66
Issue number	SUPPL. 1
DOIs	https://doi.org/10.1007/s003390051205
State	Published - 1998
Externally published	Yes

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The AFMas a tool for chromosomal dissection - The influence of physical parameters

Abstract

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