Technique to assess gene function in hop (humulus lupulus l.)-Powdery mildew interactions

K. Oberhollenzer, E. Seigner, R. Eichmann, R. Hückelhoven

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations


Improving resistance to powdery mildew (Podosphaera macularis ssp. humuli, Pm) is a major objective in hop breeding. To achieve this, classical breeding can be assisted by marker-assisted breeding or by transgenic approaches. For both these techniques, it would be of advantage to have DNA sequences available with confirmed function in the hop-powdery mildew interaction. Here, we report on the adaptation of a transient transformation assay for the functional assessment of defence-or susceptibility-related genes in hop as already available for barley and wheat. As a proof of concept, we performed transient-induced gene silencing (TIGS) of a putative hop powdery mildew susceptibility factor in hair cells, which resulted in a significantly reduced penetration success of the powdery mildew fungus.

Original languageEnglish
Title of host publicationIII International Humulus Symposium
PublisherInternational Society for Horticultural Science
Number of pages8
ISBN (Print)9789066056961
StatePublished - 25 Oct 2013

Publication series

NameActa Horticulturae
ISSN (Print)0567-7572


  • Defence-related genes
  • Hop breeding
  • Podosphaera macularis
  • Resistance
  • Transient transformation assay
  • Transient-induced gene silencing (TIGS)


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