TY - JOUR
T1 - Targeted LC-MS/MS Profiling of Bile Acids in Various Animal Tissues
AU - Reiter, Sinah
AU - Dunkel, Andreas
AU - Dawid, Corinna
AU - Hofmann, Thomas
N1 - Publisher Copyright:
© 2021 The Authors. Published by American Chemical Society
PY - 2021/9/15
Y1 - 2021/9/15
N2 - Bile acids are being increasingly investigated in humans and laboratory animals as markers for various diseases in addition to their important functions, such as promoting the emulsification in fat digestion and preventing gallstone formation. In humans and animals, primary bile acids are formed from cholesterol in the liver, converted in the intestine into various secondary bile acids by the intestinal microbiota and reabsorbed in the terminal ileum, and partially returned to the liver. A universal high-throughput workflow, including a simple workup, was applied as a tool for bile acid analysis in animal studies. The complex bile acid profiles in various tissues, organs, and body fluids from different animals were mapped using a newly developed comprehensive liquid chromatography-tandem mass spectrometry method. The method can also be used in screening food to obtain information about the nutritional content of bile acids. This could be relevant to investigations on various animal diseases and on the bioavailability of bile acids that pass through the gastric tract.
AB - Bile acids are being increasingly investigated in humans and laboratory animals as markers for various diseases in addition to their important functions, such as promoting the emulsification in fat digestion and preventing gallstone formation. In humans and animals, primary bile acids are formed from cholesterol in the liver, converted in the intestine into various secondary bile acids by the intestinal microbiota and reabsorbed in the terminal ileum, and partially returned to the liver. A universal high-throughput workflow, including a simple workup, was applied as a tool for bile acid analysis in animal studies. The complex bile acid profiles in various tissues, organs, and body fluids from different animals were mapped using a newly developed comprehensive liquid chromatography-tandem mass spectrometry method. The method can also be used in screening food to obtain information about the nutritional content of bile acids. This could be relevant to investigations on various animal diseases and on the bioavailability of bile acids that pass through the gastric tract.
KW - UHPLC-ESI-LC-MS/MS
KW - animal tissue
KW - bile acid distribution
KW - bile acids
KW - targeted metabolite profiling
UR - http://www.scopus.com/inward/record.url?scp=85115617056&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.1c03433
DO - 10.1021/acs.jafc.1c03433
M3 - Article
C2 - 34490775
AN - SCOPUS:85115617056
SN - 0021-8561
VL - 69
SP - 10572
EP - 10580
JO - Journal of agricultural and food chemistry
JF - Journal of agricultural and food chemistry
IS - 36
ER -