Targeted LC-MS/MS Profiling of Bile Acids in Various Animal Tissues

Sinah Reiter, Andreas Dunkel, Corinna Dawid, Thomas Hofmann

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Bile acids are being increasingly investigated in humans and laboratory animals as markers for various diseases in addition to their important functions, such as promoting the emulsification in fat digestion and preventing gallstone formation. In humans and animals, primary bile acids are formed from cholesterol in the liver, converted in the intestine into various secondary bile acids by the intestinal microbiota and reabsorbed in the terminal ileum, and partially returned to the liver. A universal high-throughput workflow, including a simple workup, was applied as a tool for bile acid analysis in animal studies. The complex bile acid profiles in various tissues, organs, and body fluids from different animals were mapped using a newly developed comprehensive liquid chromatography-tandem mass spectrometry method. The method can also be used in screening food to obtain information about the nutritional content of bile acids. This could be relevant to investigations on various animal diseases and on the bioavailability of bile acids that pass through the gastric tract.

Original languageEnglish
Pages (from-to)10572-10580
Number of pages9
JournalJournal of agricultural and food chemistry
Volume69
Issue number36
DOIs
StatePublished - 15 Sep 2021

Keywords

  • UHPLC-ESI-LC-MS/MS
  • animal tissue
  • bile acid distribution
  • bile acids
  • targeted metabolite profiling

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