Target and identify: Triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy

Jonas Lohse; Alexandra Schindl; Natasha Danda; Chris P. Williams; Karl Kramer; Bernhard Kuster; Martin D. Witte; Guillaume Médard

doi:10.1039/c7cc07001b

Target and identify: Triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy

Jonas Lohse, Alexandra Schindl, Natasha Danda, Chris P. Williams, Karl Kramer, Bernhard Kuster, Martin D. Witte, Guillaume Médard

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4 Scopus citations

Abstract

A method for identifying probe modification of proteins via tandem mass spectrometry was developed. Azide bearing molecules are immobilized on functionalised sepharose beads via copper catalysed Huisgen-type click chemistry and selectively released under acidic conditions by chemical cleavage of the triazene linkage. We applied this method to identify the modification site of targeted-diazotransfer on BirA.

Original language	English
Pages (from-to)	11929-11932
Number of pages	4
Journal	Chemical Communications
Volume	53
Issue number	87
DOIs	https://doi.org/10.1039/c7cc07001b
State	Published - 2017

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abstract = "A method for identifying probe modification of proteins via tandem mass spectrometry was developed. Azide bearing molecules are immobilized on functionalised sepharose beads via copper catalysed Huisgen-type click chemistry and selectively released under acidic conditions by chemical cleavage of the triazene linkage. We applied this method to identify the modification site of targeted-diazotransfer on BirA.",

author = "Jonas Lohse and Alexandra Schindl and Natasha Danda and Williams, {Chris P.} and Karl Kramer and Bernhard Kuster and Witte, {Martin D.} and Guillaume M{\'e}dard",

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doi = "10.1039/c7cc07001b",

language = "English",

volume = "53",

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T2 - Triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy

AU - Lohse, Jonas

AU - Schindl, Alexandra

AU - Danda, Natasha

AU - Williams, Chris P.

AU - Kramer, Karl

AU - Kuster, Bernhard

AU - Witte, Martin D.

AU - Médard, Guillaume

PY - 2017

Y1 - 2017

N2 - A method for identifying probe modification of proteins via tandem mass spectrometry was developed. Azide bearing molecules are immobilized on functionalised sepharose beads via copper catalysed Huisgen-type click chemistry and selectively released under acidic conditions by chemical cleavage of the triazene linkage. We applied this method to identify the modification site of targeted-diazotransfer on BirA.

AB - A method for identifying probe modification of proteins via tandem mass spectrometry was developed. Azide bearing molecules are immobilized on functionalised sepharose beads via copper catalysed Huisgen-type click chemistry and selectively released under acidic conditions by chemical cleavage of the triazene linkage. We applied this method to identify the modification site of targeted-diazotransfer on BirA.

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M3 - Article

C2 - 29046906

AN - SCOPUS:85032616227

SN - 1359-7345

VL - 53

SP - 11929

EP - 11932

JO - Chemical Communications

JF - Chemical Communications

IS - 87

ER -

Target and identify: Triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy

Abstract

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