T-cell regulation in chronic paranasal sinus disease

Nicholas Van Bruaene, Claudina Angela Pérez-Novo, Tomasz M. Basinski, Thibaut Van Zele, Gabriele Holtappels, Natalie De Ruyck, Carsten Schmidt-Weber, Cezmi Akdis, Paul Van Cauwenberge, Claus Bachert, Philippe Gevaert

Research output: Contribution to journalArticlepeer-review

309 Scopus citations

Abstract

Background: Chronic rhinosinusitis is an inflammatory disease with distinct cytokine and remodeling patterns. Chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by a TH2-skewed eosinophilic inflammation, whereas chronic rhinosinusitis without nasal polyps (CRSsNP) represents a predominant TH1 milieu. Objective: We aimed to study the direct tissue expression of transcription factors for T-cell subpopulations, including T regulatory cells, in relation to the cytokine expression patterns in the different disease subgroups. Methods: The expression of forkhead box P3 (FOXP3), T-box transcription factor (T-bet), GATA-3, retinoid acid-related orphan receptor C (RORc), the suppressive cytokines TGF-β1 and IL-10, and TH1/ TH2/ TH17 cytokines (IFN-γ, IL-4, IL-5, IL-13, IL-17) were analyzed by means of RT-PCR in 13 CRSsNP, 16 CRSwNP, and 10 control samples. Additional protein measurements were performed for TGF-β1 and IFN-γ. Results: In CRSwNP, we observed a significantly lower FOXP3 mRNA and TGF-β1 protein expression, but a significantly higher T-bet, GATA-3, IL-5, and IL-13 mRNA expression compared with controls, whereas RORc was not significantly different compared with controls. In CRSsNP, FOXP3, T-bet, GATA-3, and RORc expression was not significantly different from controls, whereas TGF-β1 mRNA, IFN-γ mRNA, and protein were significantly higher in CRSsNP compared with controls. For IL-17, no significant differences were noted among all groups. Conclusion: We demonstrate for the first time a decreased FOXP3 expression accompanied by an upregulation of T-bet and GATA-3 and a downregulation of TGF-β1 in CRSwNP versus controls and CRSsNP.

Original languageEnglish
Pages (from-to)1435-1441.e3
JournalJournal of Allergy and Clinical Immunology
Volume121
Issue number6
DOIs
StatePublished - Jun 2008
Externally publishedYes

Keywords

  • Chronic rhinosinusitis
  • FOXP3
  • T regulatory cell
  • T-cell polarization
  • TGF-β1
  • nasal polyps
  • transcription factors

Fingerprint

Dive into the research topics of 'T-cell regulation in chronic paranasal sinus disease'. Together they form a unique fingerprint.

Cite this