Szilázsok roquefortin C és mikofenolsavtartalmának kimutatása és ennek állategészségügyi jelentosége

Translated title of the contribution: Roquefortine C and mycophenolic acid in silages: Significance for animal health

Johann Bauer, Isabell Schneweis, Karsten Meyer

Research output: Contribution to journalArticlepeer-review

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Silages play a more and more important role in animal nutrition. Due to deficient production and storage techniques, silages are contaminated in many cases with moulds of the genera Monascus, Aspergillus and Penicillium. As P. roqueforti is one of the most frequently isolated species in silages, the authors studied the natural occurrence and the animal health significance of roquefortin C and mycophenolic acid. Roquefortine C, an indole-alkaloid of the 2,5-diketopiperazine-type, was detectable in 24 out of 111 silages analysed by HPLC/UV-detector; the concentrations found ranged from 48 to 28 150 μg/kg. To evaluate the toxicological relevance of this result, a feeding experiment with female sheep was carried out. The administration of roquefortin C equivalent to 5 and 25 mg/kg silage (0,13 and 0,62 mg/kg bodyweight) over a period of 16 to 18 days led to no clinical signs of intoxication. Neither clinico-chemical (ALT, AST, GLDH, bilirubin, blood glucose) nor hematological parameters (number of erythrocytes, leucocytes and thrombocytes; hemoglobin, packed cell volume) were changed by roquefortine. The profiles of LH, FSH and progesterone during the experimental period were similar to those of the control period. But the pH of the rumen fluid decreased significantly (up to 0.5 units); this may be due to a shift of the population of rumen microorganisms caused by the antibiotic properties of roquefortin C. The post-mortem pathology including histopathology revealed no liaisons which could be attributed to the administered roquefortine C. As in addition no genotoxic properties could be detected in other testsystems, the toxicity of roquefortin C is estimated to be very low. Mycophenolic acid is a weak organic acid with antifungal, antibacterial and antiviral activities. This compound can be detected frequently in silages: concentrations between 20 and 35 000 μg/kg were analysed by LC-MS in 74 out of 233 silage-samples. The acute toxicity of mycophenolic acid is considered to be low. But this compound is a noncompetitive inhibitor of eukaryotic inosine monophosphate dehydrogenase and blocks the proliferative response of B- and T-lymphocytes as well as antibody formation. As this compound has well-known immunosuppressive properties, feeding with contaminated silage may promote the development of infectious diseases in livestock.

Translated title of the contributionRoquefortine C and mycophenolic acid in silages: Significance for animal health
Original languageHungarian
Pages (from-to)679-685
Number of pages7
JournalMagyar Allatorvosok Lapja
Issue number11
StatePublished - 2001


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