TY - JOUR
T1 - Systemic inflammatory effects of traumatic brain injury, femur fracture, and shock
T2 - An experimental murine polytrauma model
AU - Probst, C.
AU - Mirzayan, M. J.
AU - Mommsen, P.
AU - Zeckey, C.
AU - Tegeder, T.
AU - Geerken, L.
AU - Maegele, M.
AU - Samii, A.
AU - Van Griensven, M.
PY - 2012
Y1 - 2012
N2 - Objective. Despite broad research in neurotrauma and shock, little is known on systemic inflammatory effects of the clinically most relevant combined polytrauma. Experimental investigation in an animal model may provide relevant insight for therapeutic strategies. We describe the effects of a combined injury with respect to lymphocyte population and cytokine activation. Methods. 45 male C57BL/6J mice (mean weight 27g) were anesthetized with ketamine/xylazine. Animals were subjected to a weight drop closed traumatic brain injury (WD-TBI), a femoral fracture and hemorrhagic shock (FX-SH). Animals were subdivided into WD-TBI, FX-SH and combined trauma (CO-TX) groups. Subjects were sacrificed at 96h. Blood was analysed for cytokines and by flow cytometry for lymphocyte populations. Results. Mortality was 8, 13 and 47 for FX-SH, WD-TBI and CO-TX groups (P<0.05). TNF (11/13/139 for FX-SH/WD-TBI/CO-TX; P<0.05), CCL2 (78/96/227; P<0.05) and IL-6 (16/48/281; P=0.05) showed significant increases in the CO-TX group. Lymphocyte populations results for FX-SH, WD-TBI and CO-TX were: CD-4 (31/21/22; P= n.s.), CD-8 (7/28/34, P<0.05), CD-4-CD-8 (11/12/18; P= n.s.), CD-56 (36/7/8; P<0.05). Conclusion. This study shows that a combination of closed TBI and femur-fracture/ shock results in an increase of the humoral inflammation. More attention to combined injury models in inflammation research is indicated.
AB - Objective. Despite broad research in neurotrauma and shock, little is known on systemic inflammatory effects of the clinically most relevant combined polytrauma. Experimental investigation in an animal model may provide relevant insight for therapeutic strategies. We describe the effects of a combined injury with respect to lymphocyte population and cytokine activation. Methods. 45 male C57BL/6J mice (mean weight 27g) were anesthetized with ketamine/xylazine. Animals were subjected to a weight drop closed traumatic brain injury (WD-TBI), a femoral fracture and hemorrhagic shock (FX-SH). Animals were subdivided into WD-TBI, FX-SH and combined trauma (CO-TX) groups. Subjects were sacrificed at 96h. Blood was analysed for cytokines and by flow cytometry for lymphocyte populations. Results. Mortality was 8, 13 and 47 for FX-SH, WD-TBI and CO-TX groups (P<0.05). TNF (11/13/139 for FX-SH/WD-TBI/CO-TX; P<0.05), CCL2 (78/96/227; P<0.05) and IL-6 (16/48/281; P=0.05) showed significant increases in the CO-TX group. Lymphocyte populations results for FX-SH, WD-TBI and CO-TX were: CD-4 (31/21/22; P= n.s.), CD-8 (7/28/34, P<0.05), CD-4-CD-8 (11/12/18; P= n.s.), CD-56 (36/7/8; P<0.05). Conclusion. This study shows that a combination of closed TBI and femur-fracture/ shock results in an increase of the humoral inflammation. More attention to combined injury models in inflammation research is indicated.
UR - http://www.scopus.com/inward/record.url?scp=84861059280&partnerID=8YFLogxK
U2 - 10.1155/2012/136020
DO - 10.1155/2012/136020
M3 - Article
C2 - 22529516
AN - SCOPUS:84861059280
SN - 0962-9351
VL - 2012
JO - Mediators of Inflammation
JF - Mediators of Inflammation
M1 - 136020
ER -