Synthetic Methods for the Preparation of a Functional Analogue of Ru360, a Potent Inhibitor of Mitochondrial Calcium Uptake

Sarah R. Nathan; Nicholas W. Pino; Daniela M. Arduino; Fabiana Perocchi; Samantha N. MacMillan; Justin J. Wilson

doi:10.1021/acs.inorgchem.6b03108

Synthetic Methods for the Preparation of a Functional Analogue of Ru360, a Potent Inhibitor of Mitochondrial Calcium Uptake

Sarah R. Nathan, Nicholas W. Pino, Daniela M. Arduino, Fabiana Perocchi, Samantha N. MacMillan, Justin J. Wilson

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

The mixed-valent oxo-bridged ruthenium complex [(HCO₂)(NH₃)₄Ru(μ-O)Ru(NH₃)₄(O₂CH)]³⁺, known as Ru360, is a selective inhibitor of mitochondrial calcium uptake. Although this compound is useful for studying the role of mitochondrial calcium in biological processes, its widespread availability is limited because of challenges in purification and characterization. Here, we describe our investigations of three different synthetic methods for the preparation of a functional analogue of this valuable compound. We demonstrate that this analogue, isolated from our procedures, exhibits potent mitochondrial calcium uptake inhibitory properties in permeabilized HeLa cells and in isolated mitochondria.

Original language	English
Pages (from-to)	3123-3126
Number of pages	4
Journal	Inorganic Chemistry
Volume	56
Issue number	6
DOIs	https://doi.org/10.1021/acs.inorgchem.6b03108
State	Published - 20 Mar 2017
Externally published	Yes

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title = "Synthetic Methods for the Preparation of a Functional Analogue of Ru360, a Potent Inhibitor of Mitochondrial Calcium Uptake",

abstract = "The mixed-valent oxo-bridged ruthenium complex [(HCO2)(NH3)4Ru(μ-O)Ru(NH3)4(O2CH)]3+, known as Ru360, is a selective inhibitor of mitochondrial calcium uptake. Although this compound is useful for studying the role of mitochondrial calcium in biological processes, its widespread availability is limited because of challenges in purification and characterization. Here, we describe our investigations of three different synthetic methods for the preparation of a functional analogue of this valuable compound. We demonstrate that this analogue, isolated from our procedures, exhibits potent mitochondrial calcium uptake inhibitory properties in permeabilized HeLa cells and in isolated mitochondria.",

author = "Nathan, {Sarah R.} and Pino, {Nicholas W.} and Arduino, {Daniela M.} and Fabiana Perocchi and MacMillan, {Samantha N.} and Wilson, {Justin J.}",

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AU - Nathan, Sarah R.

AU - Pino, Nicholas W.

AU - Arduino, Daniela M.

AU - Perocchi, Fabiana

AU - MacMillan, Samantha N.

AU - Wilson, Justin J.

PY - 2017/3/20

Y1 - 2017/3/20

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AB - The mixed-valent oxo-bridged ruthenium complex [(HCO2)(NH3)4Ru(μ-O)Ru(NH3)4(O2CH)]3+, known as Ru360, is a selective inhibitor of mitochondrial calcium uptake. Although this compound is useful for studying the role of mitochondrial calcium in biological processes, its widespread availability is limited because of challenges in purification and characterization. Here, we describe our investigations of three different synthetic methods for the preparation of a functional analogue of this valuable compound. We demonstrate that this analogue, isolated from our procedures, exhibits potent mitochondrial calcium uptake inhibitory properties in permeabilized HeLa cells and in isolated mitochondria.

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JO - Inorganic Chemistry

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Synthetic Methods for the Preparation of a Functional Analogue of Ru360, a Potent Inhibitor of Mitochondrial Calcium Uptake

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