Synthesis of spin-labeled RNAs for long range distance measurements by peldor

Nelly Piton, Olav Schiemann, Yuguang Mu, Gerhard Stock, Thomas Prisner, Joachim W. Engels

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Site directed spin labeled RNA duplexes with different interspin distances were synthesized. The radical 2,2,5,5-tetramethyl-pyrrolin-1-yloxyl-3-acetylene (TPA) was introduced during the solid-phase synthesis through a Sonogashira cross-coupling with 5-iodo-uridine. T m and CD studies showed that the spin label does not to disturb significantly the A-form of these duplexes. 4-Pulse Electron Double Resonance (PELDOR) was then used to measure intramolecular spin-spin distances of 19.3, 33.0 and 40.9 Å, which are in very good agreement with the calculated values of 17.6, 32.1 and 39.1 Å, obtained from Molecular Dynamics (MD) simulations.

Original languageEnglish
Pages (from-to)771-775
Number of pages5
JournalNucleosides, Nucleotides and Nucleic Acids
Volume24
Issue number5-7
DOIs
StatePublished - 2005
Externally publishedYes

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