Surfactin and fengycin contribute to the protection of a Bacillus subtilis strain against grape downy mildew by both direct effect and defence stimulation

Yan Li, Marie Claire Héloir, Xun Zhang, Mareen Geissler, Sophie Trouvelot, Lucile Jacquens, Marius Henkel, Xin Su, Xuewen Fang, Qi Wang, Marielle Adrian

Research output: Contribution to journalArticlepeer-review

90 Scopus citations

Abstract

Bacillus subtilis GLB191 (hereafter GLB191) is an efficient biological control agent against the biotrophic oomycete Plasmopara viticola, the causal agent of grapevine downy mildew. In this study, we show that GLB191 supernatant is also highly active against downy mildew and that the activity results from both direct effect against the pathogen and stimulation of the plant defences (induction of defence gene expression and callose production). High-performance thin-layer chromatography analysis revealed the presence of the cyclic lipopeptides fengycin and surfactin in the supernatant. Mutants affected in the production of fengycin and/or surfactin were thus obtained and allowed us to show that both surfactin and fengycin contribute to the double activity of GLB191 supernatant against downy mildew. Altogether, this study suggests that GLB191 supernatant could be used as a new biocontrol product against grapevine downy mildew.

Original languageEnglish
Pages (from-to)1037-1050
Number of pages14
JournalMolecular Plant Pathology
Volume20
Issue number8
DOIs
StatePublished - Aug 2019
Externally publishedYes

Keywords

  • Bacillus subtilis
  • Plasmopara viticola
  • defence
  • downy mildew
  • fengycin
  • lipopeptide
  • surfactin

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