TY - JOUR
T1 - Supported membranes as biofunctional interfaces and smart biosensor platforms
AU - Tanaka, Motomu
AU - Sackmann, Erich
PY - 2006/11
Y1 - 2006/11
N2 - The design of soft biocompatible and functional interfaces on solids is a challenging interdisciplinary endeavour with great potentials for scientific and biotechnnological applications. By combining modern concepts of genetic engineering and bio-organic chemistry, novel classes of recombinant proteins enable the immobilization of functional nano-machineries on solid surfaces, which can even beat functions of native biological systems. Mimetics of tissues can be fabricated by deposition of ultrathin films of biopolymers (such as cellulose or hyaluronic acid), which opens new possibilities for fundamental studies of mechanisms of cell adhesion and tissue growth. Careful adjustment of interfacial potentials using such soft interlayers can also realize the stress-free immobilization of cells for application as monitors of toxic substances and drugs. Planar models of cell surfaces can be fabricated by self-assembly of membranes on solid substrates functionalized with soft polymers, which play the generic role of cytoskeleton and glycocalix. Planar geometry enables the application of various surface sensitive techniques to study structures and dynamics of biological membranes. This review presents a survey of recent development in supported membranes, and presents some potential application of this concept in smart biosensors.
AB - The design of soft biocompatible and functional interfaces on solids is a challenging interdisciplinary endeavour with great potentials for scientific and biotechnnological applications. By combining modern concepts of genetic engineering and bio-organic chemistry, novel classes of recombinant proteins enable the immobilization of functional nano-machineries on solid surfaces, which can even beat functions of native biological systems. Mimetics of tissues can be fabricated by deposition of ultrathin films of biopolymers (such as cellulose or hyaluronic acid), which opens new possibilities for fundamental studies of mechanisms of cell adhesion and tissue growth. Careful adjustment of interfacial potentials using such soft interlayers can also realize the stress-free immobilization of cells for application as monitors of toxic substances and drugs. Planar models of cell surfaces can be fabricated by self-assembly of membranes on solid substrates functionalized with soft polymers, which play the generic role of cytoskeleton and glycocalix. Planar geometry enables the application of various surface sensitive techniques to study structures and dynamics of biological membranes. This review presents a survey of recent development in supported membranes, and presents some potential application of this concept in smart biosensors.
UR - http://www.scopus.com/inward/record.url?scp=34248399190&partnerID=8YFLogxK
U2 - 10.1002/pssa.200622464
DO - 10.1002/pssa.200622464
M3 - Article
AN - SCOPUS:34248399190
SN - 1862-6300
VL - 203
SP - 3452
EP - 3462
JO - Physica Status Solidi (A) Applications and Materials Science
JF - Physica Status Solidi (A) Applications and Materials Science
IS - 14
ER -