Subtype-specific optical action potential recordings in human induced pluripotent stem cell-derived ventricular cardiomyocytes

Alexander Goedel, Dorota M. Zawada, Fangfang Zhang, Zhifen Chen, Alessandra Moretti, Daniel Sinnecker

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Cardiomyocytes generated from human induced pluripotent stem cells (iPSC-CMs) are an emerging tool in cardiovascular research. Rather than being a homogenous population of cells, the iPSC-CMs generated by current differentiation protocols represent a mixture of cells with ventricular-, atrial-, and nodal-like phenotypes, which complicates phenotypic analyses. Here, a method to optically record action potentials specifically from ventricular-like iPSC-CMs is presented. This is achieved by lentiviral transduction with a construct in which a genetically-encoded voltage indicator is under the control of a ventricular-specific promoter element. When iPSC-CMs are transduced with this construct, the voltage sensor is expressed exclusively in ventricular-like cells, enabling subtype-specific optical membrane potential recordings using time-lapse fluorescence microscopy.

Original languageEnglish
Article numbere58134
JournalJournal of Visualized Experiments
Volume2018
Issue number139
DOIs
StatePublished - 27 Sep 2018
Externally publishedYes

Keywords

  • Action potential
  • Biology
  • Cardiomyocyte subtypes
  • Cardiomyocytes
  • Induced pluripotent stem cells
  • Issue 139
  • Optical recordings
  • Optogenetics

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