Abstract
Electron Paramagnetic Resonance (EPR) spectroscopy is a powerful method to get insight into the structures and dynamics of RNA. In order to be applicable, the RNA needs to be labelled with paramagnatic centers. These can be stable organic radicals or paramagentic metal ions. In this chapter an introduction into the different EPR methods and site directed spin labeling strategies will be given and their advantages and disadvantages discussed. Applications of these methods to ribozymes and other functional RNAs are summarized and give an overview of the state of the art in the field.
| Original language | English |
|---|---|
| Title of host publication | Ribozymes |
| Subtitle of host publication | Volume 1 and Volume 2 |
| Publisher | wiley |
| Pages | 817-859 |
| Number of pages | 43 |
| Volume | 1-2 |
| ISBN (Electronic) | 9783527814527 |
| ISBN (Print) | 9783527344543 |
| DOIs | |
| State | Published - 1 Jan 2021 |
| Externally published | Yes |