Studies on the structure of replicative intermediates in bacteriophage M 13 single stranded DNA synthesis

Barbara E. Kessler-liebscher; Walter L. Staudenbauer; Peter Hans Hofschneider

doi:10.1093/nar/2.1.131

Studies on the structure of replicative intermediates in bacteriophage M 13 single stranded DNA synthesis

Barbara E. Kessler-liebscher, Walter L. Staudenbauer, Peter Hans Hofschneider

Max Planck Institute of Biochemistry

Research output: Contribution to journal › Article › peer-review

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Abstract

Pulse-labeled replicative intermediates in M 13 single stranded DNA synthesis can be separated by dye-buoyant density centrifugation into two major fractions: Supercoiled molecules (RI I) containing viral strands of more than one genome length, and "relaxed" molecules (RI II) with labeled DNA chains shorter than unit length. It is postulated that RI II molecules might be formed in vivo by site-specific nicking of RF I molecules.

Original language	English
Pages (from-to)	131-142
Number of pages	12
Journal	Nucleic Acids Research
Volume	2
Issue number	1
DOIs	https://doi.org/10.1093/nar/2.1.131
State	Published - Jan 1975
Externally published	Yes

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AB - Pulse-labeled replicative intermediates in M 13 single stranded DNA synthesis can be separated by dye-buoyant density centrifugation into two major fractions: Supercoiled molecules (RI I) containing viral strands of more than one genome length, and "relaxed" molecules (RI II) with labeled DNA chains shorter than unit length. It is postulated that RI II molecules might be formed in vivo by site-specific nicking of RF I molecules.

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Studies on the structure of replicative intermediates in bacteriophage M 13 single stranded DNA synthesis

Abstract

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