Studies on the spectrophotometric determination of DNA hybridization from renaturation rates

Volker A.R. Huss; Herbert Festl; Karl Heinz Schleifer

doi:10.1016/S0723-2020(83)80048-4

Studies on the spectrophotometric determination of DNA hybridization from renaturation rates

Volker A.R. Huss
, Herbert Festl
, Karl Heinz Schleifer

TUM Emeriti of Excellence

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

1590 Scopus citations

Abstract

The optical method of De Ley et al. (1970) for determining DNA/DNA homologies was reexamined. Several parameters such as incubation temperature, incubation time, DNA concentration and DNA fragment length were checked and the optimal conditions established. Hybridization data of several anaerobic Gram-positive cocci were compared with values obtained by the membrane filter technique. The agreement is excellent above a degree of binding of 25–30%.

Original language	English
Pages (from-to)	184-192
Number of pages	9
Journal	Systematic and Applied Microbiology
Volume	4
Issue number	2
DOIs	https://doi.org/10.1016/S0723-2020(83)80048-4
State	Published - 1983

Keywords

DNA/DNA hybridization
Optical determination of DNA renaturation rates

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10.1016/S0723-2020(83)80048-4

Cite this

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title = "Studies on the spectrophotometric determination of DNA hybridization from renaturation rates",

abstract = "The optical method of De Ley et al. (1970) for determining DNA/DNA homologies was reexamined. Several parameters such as incubation temperature, incubation time, DNA concentration and DNA fragment length were checked and the optimal conditions established. Hybridization data of several anaerobic Gram-positive cocci were compared with values obtained by the membrane filter technique. The agreement is excellent above a degree of binding of 25–30\%.",

keywords = "DNA/DNA hybridization, Optical determination of DNA renaturation rates",

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year = "1983",

doi = "10.1016/S0723-2020(83)80048-4",

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volume = "4",

pages = "184--192",

journal = "Systematic and Applied Microbiology",

issn = "0723-2020",

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AU - Huss, Volker A.R.

AU - Festl, Herbert

AU - Schleifer, Karl Heinz

PY - 1983

Y1 - 1983

N2 - The optical method of De Ley et al. (1970) for determining DNA/DNA homologies was reexamined. Several parameters such as incubation temperature, incubation time, DNA concentration and DNA fragment length were checked and the optimal conditions established. Hybridization data of several anaerobic Gram-positive cocci were compared with values obtained by the membrane filter technique. The agreement is excellent above a degree of binding of 25–30%.

AB - The optical method of De Ley et al. (1970) for determining DNA/DNA homologies was reexamined. Several parameters such as incubation temperature, incubation time, DNA concentration and DNA fragment length were checked and the optimal conditions established. Hybridization data of several anaerobic Gram-positive cocci were compared with values obtained by the membrane filter technique. The agreement is excellent above a degree of binding of 25–30%.

KW - DNA/DNA hybridization

KW - Optical determination of DNA renaturation rates

UR - https://www.scopus.com/pages/publications/0020549589

U2 - 10.1016/S0723-2020(83)80048-4

DO - 10.1016/S0723-2020(83)80048-4

M3 - Article

AN - SCOPUS:0020549589

SN - 0723-2020

VL - 4

SP - 184

EP - 192

JO - Systematic and Applied Microbiology

JF - Systematic and Applied Microbiology

IS - 2

ER -

Studies on the spectrophotometric determination of DNA hybridization from renaturation rates

Abstract

Keywords

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