TY - JOUR
T1 - Studies on the spectrophotometric determination of DNA hybridization from renaturation rates
AU - Huss, Volker A.R.
AU - Festl, Herbert
AU - Schleifer, Karl Heinz
PY - 1983
Y1 - 1983
N2 - The optical method of De Ley et al. (1970) for determining DNA/DNA homologies was reexamined. Several parameters such as incubation temperature, incubation time, DNA concentration and DNA fragment length were checked and the optimal conditions established. Hybridization data of several anaerobic Gram-positive cocci were compared with values obtained by the membrane filter technique. The agreement is excellent above a degree of binding of 25–30%.
AB - The optical method of De Ley et al. (1970) for determining DNA/DNA homologies was reexamined. Several parameters such as incubation temperature, incubation time, DNA concentration and DNA fragment length were checked and the optimal conditions established. Hybridization data of several anaerobic Gram-positive cocci were compared with values obtained by the membrane filter technique. The agreement is excellent above a degree of binding of 25–30%.
KW - DNA/DNA hybridization
KW - Optical determination of DNA renaturation rates
UR - http://www.scopus.com/inward/record.url?scp=0020549589&partnerID=8YFLogxK
U2 - 10.1016/S0723-2020(83)80048-4
DO - 10.1016/S0723-2020(83)80048-4
M3 - Article
AN - SCOPUS:0020549589
SN - 0723-2020
VL - 4
SP - 184
EP - 192
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
IS - 2
ER -