Studies on the spectrophotometric determination of DNA hybridization from renaturation rates

Volker A.R. Huss, Herbert Festl, Karl Heinz Schleifer

Research output: Contribution to journalArticlepeer-review

1583 Scopus citations

Abstract

The optical method of De Ley et al. (1970) for determining DNA/DNA homologies was reexamined. Several parameters such as incubation temperature, incubation time, DNA concentration and DNA fragment length were checked and the optimal conditions established. Hybridization data of several anaerobic Gram-positive cocci were compared with values obtained by the membrane filter technique. The agreement is excellent above a degree of binding of 25–30%.

Original languageEnglish
Pages (from-to)184-192
Number of pages9
JournalSystematic and Applied Microbiology
Volume4
Issue number2
DOIs
StatePublished - 1983
Externally publishedYes

Keywords

  • DNA/DNA hybridization
  • Optical determination of DNA renaturation rates

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