TY - JOUR
T1 - Studies on the methanolysis of small amounts of purified phospholipids for gas chromatographic analysis of fatty acid methyl esters
AU - Eder, K.
AU - Reichlmayr-Lais, A. M.
AU - Kirchgessner, M.
PY - 1992/8/21
Y1 - 1992/8/21
N2 - The methanolysis of small amounts of purified phosphoglycerides and sphingomyelin was studied and a quantitative comparison of five methods for the methanolysis of standard phosphoglycerides was made. These methods were based on methanolysis with boron trifluoride-methanol, methanolic sodium methoxide (at ambient temperature and with heating) and methanolic sulphuric acid. A further method was based on saponification with methanolic sodium hydroxide and subsequent esterification with boron trifluoride- methanol. Under the experimental conditions, only the sodium methoxide-catalysed method at ambient temperature gave complete methanolysis of phosphoglycerides. For methanolysis of sphingomyelin, boron trifiuoride-methanol, methanolic sulphuric acid and methanolic hydrochloric acid were used. It was found that complete methanolysis of sphingomyelin takes 15 h at 90°C. Based on these results, procedures for the methanolysis of phosphoglycerides and sphingomyelin separated by high-performance liquid chromatography are presented.
AB - The methanolysis of small amounts of purified phosphoglycerides and sphingomyelin was studied and a quantitative comparison of five methods for the methanolysis of standard phosphoglycerides was made. These methods were based on methanolysis with boron trifluoride-methanol, methanolic sodium methoxide (at ambient temperature and with heating) and methanolic sulphuric acid. A further method was based on saponification with methanolic sodium hydroxide and subsequent esterification with boron trifluoride- methanol. Under the experimental conditions, only the sodium methoxide-catalysed method at ambient temperature gave complete methanolysis of phosphoglycerides. For methanolysis of sphingomyelin, boron trifiuoride-methanol, methanolic sulphuric acid and methanolic hydrochloric acid were used. It was found that complete methanolysis of sphingomyelin takes 15 h at 90°C. Based on these results, procedures for the methanolysis of phosphoglycerides and sphingomyelin separated by high-performance liquid chromatography are presented.
UR - http://www.scopus.com/inward/record.url?scp=0026781222&partnerID=8YFLogxK
U2 - 10.1016/0021-9673(92)87054-C
DO - 10.1016/0021-9673(92)87054-C
M3 - Article
C2 - 1447360
AN - SCOPUS:0026781222
SN - 0021-9673
VL - 607
SP - 55
EP - 67
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1
ER -