TY - JOUR
T1 - Studies on the extraction of phospholipids from erythrocyte membranes in the rat
AU - Eder, Klaus
AU - Reichlmayr-Lais, Anna Maria
AU - Kirchgeßner, Manfred
PY - 1993/10/15
Y1 - 1993/10/15
N2 - The effects of choice of solvent and period of extraction on recovery of phospholipid classes extracted from rat erythrocyte membranes were investigated. For extraction of phosphatidylserine (PS) the best recovery was achieved using chloroform-methanol (2:1. v/v), for diacyl phosphatidylethanolamine (PE) acetone-isopropanol (3:2, v/v), and for extraction of plasmalogen PE, phosphatidylcholine (PC) and sphingomyelin (SM) using hexaneisopropanol (3:2, v/v). Using hexane-isopropanol (3:2, v/v), boiling during the extraction increased the recovery of some of the individual phospholipid classes whereas mechanical treatment of the sample had only a slight effect. Furthermore, the fatty acid composition of PS and PE varied most with different extraction conditions and the phospholipid classes PC and SM were extracted more readily from rat erythrocyte membranes than PS, diacyl and plasmalogen PE.
AB - The effects of choice of solvent and period of extraction on recovery of phospholipid classes extracted from rat erythrocyte membranes were investigated. For extraction of phosphatidylserine (PS) the best recovery was achieved using chloroform-methanol (2:1. v/v), for diacyl phosphatidylethanolamine (PE) acetone-isopropanol (3:2, v/v), and for extraction of plasmalogen PE, phosphatidylcholine (PC) and sphingomyelin (SM) using hexaneisopropanol (3:2, v/v). Using hexane-isopropanol (3:2, v/v), boiling during the extraction increased the recovery of some of the individual phospholipid classes whereas mechanical treatment of the sample had only a slight effect. Furthermore, the fatty acid composition of PS and PE varied most with different extraction conditions and the phospholipid classes PC and SM were extracted more readily from rat erythrocyte membranes than PS, diacyl and plasmalogen PE.
KW - Erythrocyte membranes
KW - Extraction
KW - Fatty acids
KW - Phospholipid classes
KW - Rat
KW - Solvent
UR - http://www.scopus.com/inward/record.url?scp=0027385534&partnerID=8YFLogxK
U2 - 10.1016/0009-8981(93)90200-N
DO - 10.1016/0009-8981(93)90200-N
M3 - Article
C2 - 8306467
AN - SCOPUS:0027385534
SN - 0009-8981
VL - 219
SP - 93
EP - 104
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 1-2
ER -