Studies on the Enzymic Hydrolysis of Bound Aroma Components from Carica papaya Fruit

HRGC and HRGC-MS identifications of bound volatiles from papaya fruit (Carica papaya, L.) were achieved after isolation of an extract obtained by Amberlite XAD-2 adsorption and methanol elution followed by simultaneous enzyme catalysis extraction (SECE) using glycosidase (emulsin) and acid phosphatase. Aromatic substances, such as, e.g., benzaldehyde, benzyl alcohol, 2-phenylethanol, and benzyl isothiocyanate as well as (E)-3,7-dimethylocta-2,6-dienoic acid, were liberated by glycosidase, while the monoterpene alcohols linalool and 2,6-dimethyloct-7-ene-2,3,6-triol were released by phosphatase activity. As precursor of the phosphate-bound 2,6-dimethyloct-7-ene-2,3,6-triol, the phosphorylated 6,7-epoxylinalool is discussed.