Abstract
The final step in the biosynthesis of the 22nd genetically encoded amino acid, pyrrolysine, is catalyzed by PylD, a structurally and mechanistically unique dehydrogenase. This catalyzed reaction includes an induced-fit mechanism achieved by major structural rearrangements of the N-terminal helix upon substrate binding. Different steps of the reaction trajectory are visualized by complex structures of PylD with substrate and product.
| Original language | English |
|---|---|
| Pages (from-to) | 7033-7037 |
| Number of pages | 5 |
| Journal | Angewandte Chemie International Edition in English |
| Volume | 52 |
| Issue number | 27 |
| DOIs | |
| State | Published - 1 Jul 2013 |
Keywords
- Methanosarcina barkeri
- PylD
- amino acids
- dehydrogenases
- pyrrolysine