Structural and functional partitioning of bread wheat chromosome 3B

Frédéric Choulet; Adriana Alberti; Sébastien Theil; Natasha Glover; Valérie Barbe; Josquin Daron; Lise Pingault; Pierre Sourdille; Arnaud Couloux; Etienne Paux; Philippe Leroy; Sophie Mangenot; Nicolas Guilhot; Jacques Le Gouis; Francois Balfourier; Michael Alaux; Véronique Jamilloux; Julie Poulain; Céline Durand; Arnaud Bellec; Christine Gaspin; Jan Safar; Jaroslav Dolezel; Jane Rogers; Klaas Vandepoele; Jean Marc Aury; Klaus Mayer; Hélène Berges; Hadi Quesneville; Patrick Wincker; Catherine Feuillet

doi:10.1126/science.1249721

Structural and functional partitioning of bread wheat chromosome 3B

Frédéric Choulet
, Adriana Alberti
, Sébastien Theil
, Natasha Glover
, Valérie Barbe
, Josquin Daron
, Lise Pingault
, Pierre Sourdille
, Arnaud Couloux
, Etienne Paux
, Philippe Leroy
, Sophie Mangenot
, Nicolas Guilhot
, Jacques Le Gouis
, Francois Balfourier
, Michael Alaux
, Véronique Jamilloux
, Julie Poulain
, Céline Durand
, Arnaud Bellec

Christine Gaspin, Jan Safar, Jaroslav Dolezel, Jane Rogers, Klaas Vandepoele, Jean Marc Aury, Klaus Mayer, Hélène Berges, Hadi Quesneville, Patrick Wincker, Catherine Feuillet

Research output: Contribution to journal › Article › peer-review

479 Scopus citations

Abstract

We produced a reference sequence of the 1-gigabase chromosome 3B of hexaploid bread wheat. By sequencing 8452 bacterial artificial chromosomes in pools, we assembled a sequence of 774 megabases carrying 5326 protein-coding genes, 1938 pseudogenes, and 85% of transposable elements. The distribution of structural and functional features along the chromosome revealed partitioning correlated with meiotic recombination. Comparative analyses indicated high wheat-specific inter- and intrachromosomal gene duplication activities that are potential sources of variability for adaption. In addition to providing a better understanding of the organization, function, and evolution of a large and polyploid genome, the availability of a high-quality sequence anchored to genetic maps will accelerate the identification of genes underlying important agronomic traits.

Original language	English
Article number	1249721
Journal	Science
Volume	345
Issue number	6194
DOIs	https://doi.org/10.1126/science.1249721
State	Published - 2014
Externally published	Yes

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Choulet, F., Alberti, A., Theil, S., Glover, N., Barbe, V., Daron, J., Pingault, L., Sourdille, P., Couloux, A., Paux, E., Leroy, P., Mangenot, S., Guilhot, N., Le Gouis, J., Balfourier, F., Alaux, M., Jamilloux, V., Poulain, J., Durand, C., ... Feuillet, C. (2014). Structural and functional partitioning of bread wheat chromosome 3B. Science, 345(6194), Article 1249721. https://doi.org/10.1126/science.1249721

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title = "Structural and functional partitioning of bread wheat chromosome 3B",

abstract = "We produced a reference sequence of the 1-gigabase chromosome 3B of hexaploid bread wheat. By sequencing 8452 bacterial artificial chromosomes in pools, we assembled a sequence of 774 megabases carrying 5326 protein-coding genes, 1938 pseudogenes, and 85\% of transposable elements. The distribution of structural and functional features along the chromosome revealed partitioning correlated with meiotic recombination. Comparative analyses indicated high wheat-specific inter- and intrachromosomal gene duplication activities that are potential sources of variability for adaption. In addition to providing a better understanding of the organization, function, and evolution of a large and polyploid genome, the availability of a high-quality sequence anchored to genetic maps will accelerate the identification of genes underlying important agronomic traits.",

author = "Fr{\'e}d{\'e}ric Choulet and Adriana Alberti and S{\'e}bastien Theil and Natasha Glover and Val{\'e}rie Barbe and Josquin Daron and Lise Pingault and Pierre Sourdille and Arnaud Couloux and Etienne Paux and Philippe Leroy and Sophie Mangenot and Nicolas Guilhot and \{Le Gouis\}, Jacques and Francois Balfourier and Michael Alaux and V{\'e}ronique Jamilloux and Julie Poulain and C{\'e}line Durand and Arnaud Bellec and Christine Gaspin and Jan Safar and Jaroslav Dolezel and Jane Rogers and Klaas Vandepoele and Aury, \{Jean Marc\} and Klaus Mayer and H{\'e}l{\`e}ne Berges and Hadi Quesneville and Patrick Wincker and Catherine Feuillet",

year = "2014",

doi = "10.1126/science.1249721",

language = "English",

volume = "345",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "6194",

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Choulet, F, Alberti, A, Theil, S, Glover, N, Barbe, V, Daron, J, Pingault, L, Sourdille, P, Couloux, A, Paux, E, Leroy, P, Mangenot, S, Guilhot, N, Le Gouis, J, Balfourier, F, Alaux, M, Jamilloux, V, Poulain, J, Durand, C, Bellec, A, Gaspin, C, Safar, J, Dolezel, J, Rogers, J, Vandepoele, K, Aury, JM, Mayer, K, Berges, H, Quesneville, H, Wincker, P & Feuillet, C 2014, 'Structural and functional partitioning of bread wheat chromosome 3B', Science, vol. 345, no. 6194, 1249721. https://doi.org/10.1126/science.1249721

TY - JOUR

T1 - Structural and functional partitioning of bread wheat chromosome 3B

AU - Choulet, Frédéric

AU - Alberti, Adriana

AU - Theil, Sébastien

AU - Glover, Natasha

AU - Barbe, Valérie

AU - Daron, Josquin

AU - Pingault, Lise

AU - Sourdille, Pierre

AU - Couloux, Arnaud

AU - Paux, Etienne

AU - Leroy, Philippe

AU - Mangenot, Sophie

AU - Guilhot, Nicolas

AU - Le Gouis, Jacques

AU - Balfourier, Francois

AU - Alaux, Michael

AU - Jamilloux, Véronique

AU - Poulain, Julie

AU - Durand, Céline

AU - Bellec, Arnaud

AU - Gaspin, Christine

AU - Safar, Jan

AU - Dolezel, Jaroslav

AU - Rogers, Jane

AU - Vandepoele, Klaas

AU - Aury, Jean Marc

AU - Mayer, Klaus

AU - Berges, Hélène

AU - Quesneville, Hadi

AU - Wincker, Patrick

AU - Feuillet, Catherine

PY - 2014

Y1 - 2014

N2 - We produced a reference sequence of the 1-gigabase chromosome 3B of hexaploid bread wheat. By sequencing 8452 bacterial artificial chromosomes in pools, we assembled a sequence of 774 megabases carrying 5326 protein-coding genes, 1938 pseudogenes, and 85% of transposable elements. The distribution of structural and functional features along the chromosome revealed partitioning correlated with meiotic recombination. Comparative analyses indicated high wheat-specific inter- and intrachromosomal gene duplication activities that are potential sources of variability for adaption. In addition to providing a better understanding of the organization, function, and evolution of a large and polyploid genome, the availability of a high-quality sequence anchored to genetic maps will accelerate the identification of genes underlying important agronomic traits.

AB - We produced a reference sequence of the 1-gigabase chromosome 3B of hexaploid bread wheat. By sequencing 8452 bacterial artificial chromosomes in pools, we assembled a sequence of 774 megabases carrying 5326 protein-coding genes, 1938 pseudogenes, and 85% of transposable elements. The distribution of structural and functional features along the chromosome revealed partitioning correlated with meiotic recombination. Comparative analyses indicated high wheat-specific inter- and intrachromosomal gene duplication activities that are potential sources of variability for adaption. In addition to providing a better understanding of the organization, function, and evolution of a large and polyploid genome, the availability of a high-quality sequence anchored to genetic maps will accelerate the identification of genes underlying important agronomic traits.

UR - https://www.scopus.com/pages/publications/84904390032

U2 - 10.1126/science.1249721

DO - 10.1126/science.1249721

M3 - Article

C2 - 25035497

AN - SCOPUS:84904390032

SN - 0036-8075

VL - 345

JO - Science

JF - Science

IS - 6194

M1 - 1249721

ER -

Structural and functional partitioning of bread wheat chromosome 3B

Abstract

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