Abstract
The solvent helps out: Conventional protocols for structural analysis of protein complexes often fail if only sparse experimental data are available. NMR-spectroscopy-derived solvent paramagnetic relaxation enhancements (PREs) from a soluble spin label (picture) significantly improve the structural quality of a 150 kDa protein complex. This general protocol is an efficient approach for structural analysis of large protein complexes in solution.
| Original language | English |
|---|---|
| Pages (from-to) | 3993-3997 |
| Number of pages | 5 |
| Journal | Angewandte Chemie International Edition in English |
| Volume | 50 |
| Issue number | 17 |
| DOIs | |
| State | Published - 18 Apr 2011 |
Keywords
- NMR spectroscopy
- paramagnetic relaxation enhancement
- proteins
- structural biology