TY - JOUR
T1 - Stromelysin-3 (ST-3) mRNA expression in colorectal carcinomas
T2 - Localization and clinicopathologic correlations
AU - Thewes, M.
AU - Pohlmann, G.
AU - Atkinson, M.
AU - Mueller, J.
AU - Pütz, B.
AU - Höfler, H.
PY - 1996
Y1 - 1996
N2 - Stromelysin-3 (ST-3) mRNA expression was studied in 28 colorectal carcinomas and compared with that of adjacent nontumorous tissue. By Northern blot analysis, levels of ST-3 mRNA were significantly increased in the carcinomas compared with nontumorous tissue. No significant correlation with ST-3 expression was seen with degree of invasion, nodal or distant metastases, or histologic grade. In situ hybridization of nontumorous tissue showed no significant ST-3 expression. In tumor tissue, ST-3 mRNA was localized adjacent to colon carcinoma cells in irregular foci within the stroma. No significant difference in ST-3 expression was found between the center and periphery of the colon tumors. Most of the colon carcinomas (26 of 28) induced an expression of ST-3 in the directly adjacent stroma. No significant correlation between ST-3 mRNA expression and tumor stage and grade was seen. By Northern blot, we also saw expression of ST-3 in noncarcinomatous tissue, further supporting the concept that ST-3 expression is a tumor-induced but not a tumor-specific phenomenon.
AB - Stromelysin-3 (ST-3) mRNA expression was studied in 28 colorectal carcinomas and compared with that of adjacent nontumorous tissue. By Northern blot analysis, levels of ST-3 mRNA were significantly increased in the carcinomas compared with nontumorous tissue. No significant correlation with ST-3 expression was seen with degree of invasion, nodal or distant metastases, or histologic grade. In situ hybridization of nontumorous tissue showed no significant ST-3 expression. In tumor tissue, ST-3 mRNA was localized adjacent to colon carcinoma cells in irregular foci within the stroma. No significant difference in ST-3 expression was found between the center and periphery of the colon tumors. Most of the colon carcinomas (26 of 28) induced an expression of ST-3 in the directly adjacent stroma. No significant correlation between ST-3 mRNA expression and tumor stage and grade was seen. By Northern blot, we also saw expression of ST-3 in noncarcinomatous tissue, further supporting the concept that ST-3 expression is a tumor-induced but not a tumor-specific phenomenon.
KW - Colon carcinoma
KW - In situ hybridization
KW - Metalloproteinase
KW - Stromelysin-3
UR - http://www.scopus.com/inward/record.url?scp=0029905683&partnerID=8YFLogxK
U2 - 10.1097/00019606-199612000-00010
DO - 10.1097/00019606-199612000-00010
M3 - Article
C2 - 8955621
AN - SCOPUS:0029905683
SN - 1052-9551
VL - 5
SP - 284
EP - 290
JO - Diagnostic Molecular Pathology
JF - Diagnostic Molecular Pathology
IS - 4
ER -