TY - JOUR
T1 - Strawberry fruit FanCXE1 carboxylesterase is involved in the catabolism of volatile esters during the ripening process
AU - Martínez-Rivas, Felix Juan
AU - Blanco-Portales, Rosario
AU - Moyano, Enriqueta
AU - Alseekh, Saleh
AU - Caballero, Jose Luis
AU - Schwab, Wilfried
AU - Fernie, Alisdair R.
AU - Muñoz-Blanco, Juan
AU - Molina-Hidalgo, Francisco Javier
N1 - Publisher Copyright:
© 2022 The Author(s). Published by Oxford University Press on behalf of Nanjing Agricultural University.
PY - 2022
Y1 - 2022
N2 - Volatile compounds produced during ripening of strawberry are key determinants of fruit quality and consumer preference. Strawberry volatiles are largely esters which are synthesized by alcohol acyltransferases (AATs) and degraded by carboxylesterases (CXEs). Although CXE activity can have a marked influence on volatile contents in ripe strawberry fruits, CXE function and regulation in them are poorly known. Here, we report the biochemical and functional characterization of the fruit receptacle-specific and ripening-related carboxylesterase FanCXE1. The expression of the corresponding gene was found to be antagonistically regulated by auxins and abscisic acid, key hormones that regulate fruit growth and ripening in strawberry. In vitro, FanCXE1 was able to hydrolyze artificial ester substrates similar to those produced by ripe strawberry fruits. Transient suppression of the FanCXE1 gene by RNAi resulted in an increase of important volatile esters such as methyl hexanoate, methyl butanoate and ethyl hexanoate as well as a decrease of the alcohols hexenol and linanool. The results of this work enhance our understanding of the molecular basis for volatile syntheses and facilitate production of better flavored strawberry fruits by introduction of the relevant alleles into common cultivars.
AB - Volatile compounds produced during ripening of strawberry are key determinants of fruit quality and consumer preference. Strawberry volatiles are largely esters which are synthesized by alcohol acyltransferases (AATs) and degraded by carboxylesterases (CXEs). Although CXE activity can have a marked influence on volatile contents in ripe strawberry fruits, CXE function and regulation in them are poorly known. Here, we report the biochemical and functional characterization of the fruit receptacle-specific and ripening-related carboxylesterase FanCXE1. The expression of the corresponding gene was found to be antagonistically regulated by auxins and abscisic acid, key hormones that regulate fruit growth and ripening in strawberry. In vitro, FanCXE1 was able to hydrolyze artificial ester substrates similar to those produced by ripe strawberry fruits. Transient suppression of the FanCXE1 gene by RNAi resulted in an increase of important volatile esters such as methyl hexanoate, methyl butanoate and ethyl hexanoate as well as a decrease of the alcohols hexenol and linanool. The results of this work enhance our understanding of the molecular basis for volatile syntheses and facilitate production of better flavored strawberry fruits by introduction of the relevant alleles into common cultivars.
UR - http://www.scopus.com/inward/record.url?scp=85135684943&partnerID=8YFLogxK
U2 - 10.1093/hr/uhac095
DO - 10.1093/hr/uhac095
M3 - Article
AN - SCOPUS:85135684943
SN - 2662-6810
VL - 9
JO - Horticulture Research
JF - Horticulture Research
M1 - uhac095
ER -