Strategies for prokaryotic expression of eukaryotic membrane proteins

R. Laage; D. Langosch

doi:10.1034/j.1600-0854.2001.020204.x

Strategies for prokaryotic expression of eukaryotic membrane proteins

R. Laage
, D. Langosch

Heidelberg University

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

High-level heterologous expression of integral membrane proteins at full-length is a useful tool for their structural and functional characterization. Here, systems that have previously been used for efficient bacterial expression of eukaryotic membrane proteins are reviewed and novel vectors consisting of a modular fusion moiety based on nuclease A from Staphylococcus aureus are presented.

Original language	English
Pages (from-to)	99-104
Number of pages	6
Journal	Traffic
Volume	2
Issue number	2
DOIs	https://doi.org/10.1034/j.1600-0854.2001.020204.x
State	Published - 2001
Externally published	Yes

Keywords

E. coli
Expression
SNARE
Synaptobrevin
Syntaxin
Transmembrane segment

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10.1034/j.1600-0854.2001.020204.x

Cite this

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title = "Strategies for prokaryotic expression of eukaryotic membrane proteins",

abstract = "High-level heterologous expression of integral membrane proteins at full-length is a useful tool for their structural and functional characterization. Here, systems that have previously been used for efficient bacterial expression of eukaryotic membrane proteins are reviewed and novel vectors consisting of a modular fusion moiety based on nuclease A from Staphylococcus aureus are presented.",

keywords = "E. coli, Expression, SNARE, Synaptobrevin, Syntaxin, Transmembrane segment",

author = "R. Laage and D. Langosch",

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T1 - Strategies for prokaryotic expression of eukaryotic membrane proteins

AU - Laage, R.

AU - Langosch, D.

PY - 2001

Y1 - 2001

N2 - High-level heterologous expression of integral membrane proteins at full-length is a useful tool for their structural and functional characterization. Here, systems that have previously been used for efficient bacterial expression of eukaryotic membrane proteins are reviewed and novel vectors consisting of a modular fusion moiety based on nuclease A from Staphylococcus aureus are presented.

AB - High-level heterologous expression of integral membrane proteins at full-length is a useful tool for their structural and functional characterization. Here, systems that have previously been used for efficient bacterial expression of eukaryotic membrane proteins are reviewed and novel vectors consisting of a modular fusion moiety based on nuclease A from Staphylococcus aureus are presented.

KW - E. coli

KW - Expression

KW - SNARE

KW - Synaptobrevin

KW - Syntaxin

KW - Transmembrane segment

UR - https://www.scopus.com/pages/publications/0035099425

U2 - 10.1034/j.1600-0854.2001.020204.x

DO - 10.1034/j.1600-0854.2001.020204.x

M3 - Article

C2 - 11247308

AN - SCOPUS:0035099425

SN - 1398-9219

VL - 2

SP - 99

EP - 104

JO - Traffic

JF - Traffic

IS - 2

ER -

Strategies for prokaryotic expression of eukaryotic membrane proteins

Abstract

Keywords

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