Abstract
R(+)~thioctic acid is the naturally occurring cofactor in a-ketoacid, dehydrogenases. We show both photometrically by NADH + H+ oxidation and by HPLC product analysis that this enantiomer is rapidly reduced by NADH +H2 catalyzed by porcine heart lipoamide dehydrogenase/diaphorase. The racemate exhibits approximately 40% activity as compared to the R(+) form while the S(-) enantiomer photometrically shows little activity and yields no detectable reduced lipoic, acid.
| Original language | English |
|---|---|
| Pages (from-to) | 691-692 |
| Number of pages | 2 |
| Journal | Zeitschrift fur Naturforschung - Section C Journal of Biosciences |
| Volume | 49 |
| Issue number | 9-10 |
| DOIs | |
| State | Published - Oct 1994 |
Keywords
- Diaphorase
- Lipoic Acid
- Stereospecificity
- Thioctic Acid