TY - JOUR
T1 - Steady-state analysis of metabolic pathways
T2 - Comparing the double modulation method and the lin-log approach
AU - Link, Hannes
AU - Weuster-Botz, Dirk
PY - 2007/9
Y1 - 2007/9
N2 - The increasing interest in studying enzyme kinetics under in vivo conditions requires practical methods to estimate control parameters from experimental data. In contrast to currently established approaches of dynamic modelling, this paper addresses the steady-state analysis of metabolic pathways. Within the framework of metabolic control analysis (MCA), elasticity coefficients are used to describe the control properties of a local enzyme reaction. The double modulation method is one of the first experimental approaches to estimate elasticity coefficients from measurements of steady-state flux rates and metabolite concentrations. We propose a generalized form of the double modulation method and compare it to the recently developed linear-logarithmic approach.
AB - The increasing interest in studying enzyme kinetics under in vivo conditions requires practical methods to estimate control parameters from experimental data. In contrast to currently established approaches of dynamic modelling, this paper addresses the steady-state analysis of metabolic pathways. Within the framework of metabolic control analysis (MCA), elasticity coefficients are used to describe the control properties of a local enzyme reaction. The double modulation method is one of the first experimental approaches to estimate elasticity coefficients from measurements of steady-state flux rates and metabolite concentrations. We propose a generalized form of the double modulation method and compare it to the recently developed linear-logarithmic approach.
KW - Double modulation method
KW - Lin-log approach
KW - Metabolic control analysis
KW - Parameter estimation
UR - https://www.scopus.com/pages/publications/35549008920
U2 - 10.1016/j.ymben.2007.07.002
DO - 10.1016/j.ymben.2007.07.002
M3 - Article
C2 - 17889583
AN - SCOPUS:35549008920
SN - 1096-7176
VL - 9
SP - 433
EP - 441
JO - Metabolic Engineering
JF - Metabolic Engineering
IS - 5-6
ER -