Stable isotope labeling in zebrafish allows in vivo monitoring of cardiac morphogenesis

Anne Konzer, Aaron Ruhs, Helene Braun, Benno Jungblut, Thomas Braun, Marcus Krüger

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Quantitative proteomics is an important tool to study biological processes, but so far it has been challenging to apply to zebrafish. Here, we describe a large scale quantitative analysis of the zebrafish proteome using a combination of stable isotope labeling and liquid chromatography- mass spectrometry (LC-MS). Proteins derived from the fully labeled fish were used as a standard to quantify changes during embryonic heart development. LC-MS-assisted analysis of the proteome of activated leukocyte cell adhesion molecule zebrafish morphants revealed a down-regulation of components of the network required for cell adhesion and maintenance of cell shape as well as secondary changes due to arrest of cellular differentiation. Quantitative proteomics in zebrafish using the stable isotope-labeling technique provides an unprecedented resource to study developmental processes in zebrafish.

Original languageEnglish
Pages (from-to)1502-1512
Number of pages11
JournalMolecular and Cellular Proteomics
Volume12
Issue number6
DOIs
StatePublished - Jun 2013
Externally publishedYes

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