Stabilisation of immunoassays and receptor assays

B. Hock, M. Rahman, S. Rauchalles, A. Dankwardt, M. Seifert, S. Haindl, K. Kramer

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Stability of immunoassays and receptor assays depends on the integrity of antibodies and receptors as binding proteins for the analytes. In addition, coating conjugates, tracers, and if applicable, enzyme substrates have to be considered. In this study, temperature effects on monoclonal and recombinant antibodies for atrazine and on estrogen receptors were investigated in the presence and absence of stabilisers. Monoclonal antibodies for atrazine suffered only moderate activity losses after a 10-day incubation at 37°C which could be demonstrated by shifts of the calibration curves. Even more remarkable was the resistance of the analogous recombinant Fab fragments to temperatures up to 50°C. However, the estrogen receptor showed severe losses already after a 3-day exposure at 4°C. Various stabilisers were tested. In the case of antibodies, several commercial stabilisers prevented activity losses at higher temperatures. Improved stabilisation strategies are discussed. They are mainly seen in the selection of temperature-resistant recombinant antibodies and in the reduction of assay components. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)115-124
Number of pages10
JournalJournal of Molecular Catalysis B: Enzymatic
Volume7
Issue number1-4
DOIs
StatePublished - 15 Sep 1999

Keywords

  • Antibodies
  • Immunoassays
  • Receptor assays
  • Receptors
  • Stabilisation
  • Stabilisers

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