Spontaneous in vitro transformation of primary human osteoblast-like cells

Ursula Magdolen; Manfred Schmitt; Barbara Hildebrandt; Peter Diehl; Johannes Schauwecker; Belma Saldamli; Rainer Burgkart; Jutta Tübel; Rainer Gradinger; Brigitte Royer-Pokora

Spontaneous in vitro transformation of primary human osteoblast-like cells

Ursula Magdolen, Manfred Schmitt, Barbara Hildebrandt, Peter Diehl, Johannes Schauwecker, Belma Saldamli, Rainer Burgkart, Jutta Tübel, Rainer Gradinger, Brigitte Royer-Pokora

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Background: Two new tumor-like cell lines were established which developed spontaneously in vitro from normal human primary osteoblast-like cells originating from non-oncogenic bone surgery. Materials and Methods: The tumor cell properties studied included morphology, proliferation characteristics in normal and low-serum media, and anchorage-independent growth in soft agarose. Results and Conclusion: Karyotyping of the cells showed numerous rearrangements and abnormalities. These results pointed to the tumorigenic potential of the cells and demonstrate the importance of biosafety in tissue engineering and therapeutic cell applications when prolonged culture conditions are required.

Original language	English
Pages (from-to)	61-66
Number of pages	6
Journal	Cancer Genomics and Proteomics
Volume	7
Issue number	2
State	Published - 2010
Externally published	Yes

Keywords

Biosafety
Chromosome rearrangement
Immortalization
Primary human osteoblast-like cells

Cite this

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title = "Spontaneous in vitro transformation of primary human osteoblast-like cells",

abstract = "Background: Two new tumor-like cell lines were established which developed spontaneously in vitro from normal human primary osteoblast-like cells originating from non-oncogenic bone surgery. Materials and Methods: The tumor cell properties studied included morphology, proliferation characteristics in normal and low-serum media, and anchorage-independent growth in soft agarose. Results and Conclusion: Karyotyping of the cells showed numerous rearrangements and abnormalities. These results pointed to the tumorigenic potential of the cells and demonstrate the importance of biosafety in tissue engineering and therapeutic cell applications when prolonged culture conditions are required.",

keywords = "Biosafety, Chromosome rearrangement, Immortalization, Primary human osteoblast-like cells",

author = "Ursula Magdolen and Manfred Schmitt and Barbara Hildebrandt and Peter Diehl and Johannes Schauwecker and Belma Saldamli and Rainer Burgkart and Jutta T{\"u}bel and Rainer Gradinger and Brigitte Royer-Pokora",

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T1 - Spontaneous in vitro transformation of primary human osteoblast-like cells

AU - Magdolen, Ursula

AU - Schmitt, Manfred

AU - Hildebrandt, Barbara

AU - Diehl, Peter

AU - Schauwecker, Johannes

AU - Saldamli, Belma

AU - Burgkart, Rainer

AU - Tübel, Jutta

AU - Gradinger, Rainer

AU - Royer-Pokora, Brigitte

PY - 2010

Y1 - 2010

N2 - Background: Two new tumor-like cell lines were established which developed spontaneously in vitro from normal human primary osteoblast-like cells originating from non-oncogenic bone surgery. Materials and Methods: The tumor cell properties studied included morphology, proliferation characteristics in normal and low-serum media, and anchorage-independent growth in soft agarose. Results and Conclusion: Karyotyping of the cells showed numerous rearrangements and abnormalities. These results pointed to the tumorigenic potential of the cells and demonstrate the importance of biosafety in tissue engineering and therapeutic cell applications when prolonged culture conditions are required.

AB - Background: Two new tumor-like cell lines were established which developed spontaneously in vitro from normal human primary osteoblast-like cells originating from non-oncogenic bone surgery. Materials and Methods: The tumor cell properties studied included morphology, proliferation characteristics in normal and low-serum media, and anchorage-independent growth in soft agarose. Results and Conclusion: Karyotyping of the cells showed numerous rearrangements and abnormalities. These results pointed to the tumorigenic potential of the cells and demonstrate the importance of biosafety in tissue engineering and therapeutic cell applications when prolonged culture conditions are required.

KW - Biosafety

KW - Chromosome rearrangement

KW - Immortalization

KW - Primary human osteoblast-like cells

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M3 - Article

AN - SCOPUS:77950940528

SN - 1109-6535

VL - 7

SP - 61

EP - 66

JO - Cancer Genomics and Proteomics

JF - Cancer Genomics and Proteomics

IS - 2

ER -

Spontaneous in vitro transformation of primary human osteoblast-like cells

Abstract

Keywords

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