@article{815257da763c4044bbc8d1cccd58a531,
title = "Splenic red pulp macrophages are intrinsically superparamagnetic and contaminate magnetic cell isolates",
abstract = "A main function of splenic red pulp macrophages is the degradation of damaged or aged erythrocytes. Here we show that these macrophages accumulate ferrimagnetic iron oxides that render them intrinsically superparamagnetic. Consequently, these cells routinely contaminate splenic cell isolates obtained with the use of MCS, a technique that has been widely used in immunological research for decades. These contaminations can profoundly alter experimental results. In mice deficient for the transcription factor SpiC, which lack red pulp macrophages, liver Kupffer cells take over the task of erythrocyte degradation and become superparamagnetic. We describe a simple additional magnetic separation step that avoids this problem and substantially improves purity of magnetic cell isolates from the spleen.",
author = "Lars Franken and Marika Klein and Marina Spasova and Anna Elsukova and Ulf Wiedwald and Meike Welz and Percy Knolle and Michael Farle and Andreas Limmer and Christian Kurts",
note = "Funding Information: We acknowledge support by the Central Animal Facilities of the Medical Faculty Bonn and the Flow Cytometry Core Facility at the Institutes of Molecular Medicine and Experimental Immunology Bonn. Helpful discussions and experimental support in the initial stages of the magnetic measurements by U. Wiedwald and M. Acet are acknowledged. This work was supported by the Deutsche Forschungsgemeinschaft (Grants SFBTR57, SFB645, SFB704). C.K. and P.K. are members of the Excellence Cluster ImmunoSensation.",
year = "2015",
month = aug,
day = "11",
doi = "10.1038/srep12940",
language = "English",
volume = "5",
journal = "Scientific Reports",
publisher = "Nature Publishing Group",
}