Specific expression of k63-linked ubiquitination of calmodulin-like protein 5 in breast cancer of premenopausal patients

Manuel Debald, Frank Alexander Schildberg, Andrea Linke, Klaus Walgenbach, Walther Kuhn, Gunther Hartmann, Gisela Walgenbach-Brünagel

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Purpose: Posttranslational modifications such as ubiquitination regulate many functions of proteins by affecting their interaction with other molecules, their activity, and their subcellular localization. In cancer biology, the ubiquitin network has gained major interest. K63-linked ubiquitination has emerged as a posttranslational modification with functional consequences, as it acts in several processes such as protein trafficking, DNA repair, and inflammation. Moreover, k63-linked ubiquitination is involved in the regulation of carcinogenesis. Based on previous findings, the aim of this study was to evaluate the ubiquitination of CALML5 in breast cancer patients. Patients and methods: The breast cancer cell lines SkBr3, MCF7, HCC1937, and BT474 as well as 23 tumor samples of patients with primary breast cancer and the normal adjacent breast tissue were analyzed by one-dimensional immunoblot. Results: Using specific antibodies against CALML5 and k63-linked ubiquitin, we demonstrate a k63-linked ubiquitination in the nuclear fraction of premenopausal breast cancer patients. K63-linked ubiquitination of CALML5 was found in breast cancer tissue, but not found in surrounding healthy tissue. Conclusion: Our findings support the concept that ubiquitination of CALML5 in the nucleus is involved in the carcinogenesis of breast cancer in premenopausal women.

Original languageEnglish
Pages (from-to)2125-2132
Number of pages8
JournalJournal of Cancer Research and Clinical Oncology
Volume139
Issue number12
DOIs
StatePublished - Dec 2013
Externally publishedYes

Keywords

  • Breast cancer
  • CALML5
  • K63
  • Lys63
  • Ubiquitin

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