Specific detection of the gene for the extracellular neutral protease of Bacillus cereus by PCR and blot hybridization

H. J. Bach; D. Errampalli; K. T. Leung; H. Lee; A. Hartmann; J. T. Trevors; J. C. Munch

doi:10.1128/aem.65.7.3226-3228.1999

Specific detection of the gene for the extracellular neutral protease of Bacillus cereus by PCR and blot hybridization

H. J. Bach
, D. Errampalli
, K. T. Leung
, H. Lee
, A. Hartmann
, J. T. Trevors
, J. C. Munch

Helmholtz Zentrum München German Research Center for Environmental Health
University of Guelph

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A pair of primers and a gene probe for the amplification and detection of the Bacillus cereus neutral protease gene (NPRC) were developed. Specificity for the npr genes of the B. cereus group members B. cereus, B. mycoides, and B. thuringiensis was shown. Restriction polymorphism patterns of the PCR products confirmed the presence of the NPRC gene in all three species.

Original language	English
Pages (from-to)	3226-3228
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	65
Issue number	7
DOIs	https://doi.org/10.1128/aem.65.7.3226-3228.1999
State	Published - Jul 1999
Externally published	Yes

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10.1128/aem.65.7.3226-3228.1999

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title = "Specific detection of the gene for the extracellular neutral protease of Bacillus cereus by PCR and blot hybridization",

abstract = "A pair of primers and a gene probe for the amplification and detection of the Bacillus cereus neutral protease gene (NPRC) were developed. Specificity for the npr genes of the B. cereus group members B. cereus, B. mycoides, and B. thuringiensis was shown. Restriction polymorphism patterns of the PCR products confirmed the presence of the NPRC gene in all three species.",

author = "Bach, \{H. J.\} and D. Errampalli and Leung, \{K. T.\} and H. Lee and A. Hartmann and Trevors, \{J. T.\} and Munch, \{J. C.\}",

year = "1999",

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T1 - Specific detection of the gene for the extracellular neutral protease of Bacillus cereus by PCR and blot hybridization

AU - Bach, H. J.

AU - Errampalli, D.

AU - Leung, K. T.

AU - Lee, H.

AU - Hartmann, A.

AU - Trevors, J. T.

AU - Munch, J. C.

PY - 1999/7

Y1 - 1999/7

N2 - A pair of primers and a gene probe for the amplification and detection of the Bacillus cereus neutral protease gene (NPRC) were developed. Specificity for the npr genes of the B. cereus group members B. cereus, B. mycoides, and B. thuringiensis was shown. Restriction polymorphism patterns of the PCR products confirmed the presence of the NPRC gene in all three species.

AB - A pair of primers and a gene probe for the amplification and detection of the Bacillus cereus neutral protease gene (NPRC) were developed. Specificity for the npr genes of the B. cereus group members B. cereus, B. mycoides, and B. thuringiensis was shown. Restriction polymorphism patterns of the PCR products confirmed the presence of the NPRC gene in all three species.

UR - https://www.scopus.com/pages/publications/0033015226

U2 - 10.1128/aem.65.7.3226-3228.1999

DO - 10.1128/aem.65.7.3226-3228.1999

M3 - Article

C2 - 10388725

AN - SCOPUS:0033015226

SN - 0099-2240

VL - 65

SP - 3226

EP - 3228

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 7

ER -

Specific detection of the gene for the extracellular neutral protease of Bacillus cereus by PCR and blot hybridization

Abstract

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