Specific detection of the gene for the extracellular neutral protease of Bacillus cereus by PCR and blot hybridization

H. J. Bach; D. Errampalli; K. T. Leung; H. Lee; A. Hartmann; J. T. Trevors; J. C. Munch

doi:10.1128/aem.65.7.3226-3228.1999

Specific detection of the gene for the extracellular neutral protease of Bacillus cereus by PCR and blot hybridization

H. J. Bach, D. Errampalli, K. T. Leung, H. Lee, A. Hartmann, J. T. Trevors, J. C. Munch

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A pair of primers and a gene probe for the amplification and detection of the Bacillus cereus neutral protease gene (NPRC) were developed. Specificity for the npr genes of the B. cereus group members B. cereus, B. mycoides, and B. thuringiensis was shown. Restriction polymorphism patterns of the PCR products confirmed the presence of the NPRC gene in all three species.

Original language	English
Pages (from-to)	3226-3228
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	65
Issue number	7
DOIs	https://doi.org/10.1128/aem.65.7.3226-3228.1999
State	Published - Jul 1999
Externally published	Yes

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10.1128/aem.65.7.3226-3228.1999

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abstract = "A pair of primers and a gene probe for the amplification and detection of the Bacillus cereus neutral protease gene (NPRC) were developed. Specificity for the npr genes of the B. cereus group members B. cereus, B. mycoides, and B. thuringiensis was shown. Restriction polymorphism patterns of the PCR products confirmed the presence of the NPRC gene in all three species.",

author = "Bach, {H. J.} and D. Errampalli and Leung, {K. T.} and H. Lee and A. Hartmann and Trevors, {J. T.} and Munch, {J. C.}",

year = "1999",

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AU - Bach, H. J.

AU - Errampalli, D.

AU - Leung, K. T.

AU - Lee, H.

AU - Hartmann, A.

AU - Trevors, J. T.

AU - Munch, J. C.

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AB - A pair of primers and a gene probe for the amplification and detection of the Bacillus cereus neutral protease gene (NPRC) were developed. Specificity for the npr genes of the B. cereus group members B. cereus, B. mycoides, and B. thuringiensis was shown. Restriction polymorphism patterns of the PCR products confirmed the presence of the NPRC gene in all three species.

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DO - 10.1128/aem.65.7.3226-3228.1999

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ER -

Specific detection of the gene for the extracellular neutral protease of Bacillus cereus by PCR and blot hybridization

Abstract

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