Specific detection of Legionella pneumophila: Construction of a new 16S rRNA-targeted oligonucleotide probe

Dorothee Grimm, Hilde Merkert, Wolfgang Ludwig, Karl Heinz Schleifer, Jörg Hacker, Bettina C. Brand

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

Based on comparative sequence analysis, we have designed an oligonucleotide probe complementary to a region of 16S rRNA of Legionella pneumophila which allows the differentiation of L. pneumophila from other Legionella species without cultivation. The specificity of the new probe, LEGPNE1, was tested by in situ hybridization to a total of four serogroups of six strains of L. pneumophila, five different Legionella spp. and three nonlegionella species as reference strains. Furthermore, L. pneumophila cells could be easily distinguished from Legionella micdadei and Pseudomonas aeruginosa cells by using in situ hybridization with probes LEGPNE1, LEG705, and EUB338 after infection of the protozoan Acanthamoeba castellanii.

Original languageEnglish
Pages (from-to)2686-2690
Number of pages5
JournalApplied and Environmental Microbiology
Volume64
Issue number7
DOIs
StatePublished - Jul 1998
Externally publishedYes

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