Solvent engineering substantially enhances the chemoenzymatic production of surfactin

Björn Wagner; Stephan A. Sieber; Marcus Baumann; Mohamed A. Marahiel

doi:10.1002/cbic.200500495

Solvent engineering substantially enhances the chemoenzymatic production of surfactin

Björn Wagner, Stephan A. Sieber, Marcus Baumann, Mohamed A. Marahiel

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Here we report the first application of the recombinant nonribosomal peptide cyclase Srf TE as a molecular tool, optimized to operate in organic solvent (DMF) to catalyze macrocyclization of hydrophobic lipopeptides. This approach eliminates hydrolytic side-product formation and provides access to predominantly hydrophobic surfactin products.

Original language	English
Pages (from-to)	595-597
Number of pages	3
Journal	ChemBioChem
Volume	7
Issue number	4
DOIs	https://doi.org/10.1002/cbic.200500495
State	Published - Apr 2006
Externally published	Yes

Keywords

Cyclization
Enzyme catalysis
Nonribosomal peptide synthetases
Oxyanion hole
Solvent engineering

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10.1002/cbic.200500495

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abstract = "Here we report the first application of the recombinant nonribosomal peptide cyclase Srf TE as a molecular tool, optimized to operate in organic solvent (DMF) to catalyze macrocyclization of hydrophobic lipopeptides. This approach eliminates hydrolytic side-product formation and provides access to predominantly hydrophobic surfactin products.",

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AU - Sieber, Stephan A.

AU - Baumann, Marcus

AU - Marahiel, Mohamed A.

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AB - Here we report the first application of the recombinant nonribosomal peptide cyclase Srf TE as a molecular tool, optimized to operate in organic solvent (DMF) to catalyze macrocyclization of hydrophobic lipopeptides. This approach eliminates hydrolytic side-product formation and provides access to predominantly hydrophobic surfactin products.

KW - Cyclization

KW - Enzyme catalysis

KW - Nonribosomal peptide synthetases

KW - Oxyanion hole

KW - Solvent engineering

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Solvent engineering substantially enhances the chemoenzymatic production of surfactin

Abstract

Keywords

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