Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii

James M. Aramini; Yuanpeng J. Huang; John R. Cort; Sharon Goldsmith-Fischman; Rong Xiao; Liang Yu Shih; Chi K. Ho; Jinfeng Liu; Burkhard Rost; Barry Honig; Michael A. Kennedy; Thomas B. Acton; Gaetano T. Montelione

doi:10.1110/ps.03359003

Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii

James M. Aramini, Yuanpeng J. Huang, John R. Cort, Sharon Goldsmith-Fischman, Rong Xiao, Liang Yu Shih, Chi K. Ho, Jinfeng Liu, Burkhard Rost, Barry Honig, Michael A. Kennedy, Thomas B. Acton, Gaetano T. Montelione

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16 Scopus citations

Abstract

We report NMR assignments and solution structure of the 71-residue 30S ribosomal protein S28E from the archaean Pyrococcus horikoshii, target JR19 of the Northeast Structural Genomics Consortium. The structure, determined rapidly with the aid of automated backbone resonance assignment (AutoAssign) and automated structure determination (AutoStructure) software, is characterized by a four-stranded β-sheet with a classic Greek-key topology and an oligonucleotide/oligosaccharide β-barrel (OB) fold. The electrostatic surface of S28E exhibits positive and negative patches on opposite sides, the former constituting a putative binding site for RNA. The 13 C-terminal residues of the protein contain a consensus sequence motif constituting the signature of the S28E protein family. Surprisingly, this C-terminal segment is unstructured in solution.

Original language	English
Pages (from-to)	2823-2830
Number of pages	8
Journal	Protein Science
Volume	12
Issue number	12
DOIs	https://doi.org/10.1110/ps.03359003
State	Published - Dec 2003
Externally published	Yes

Keywords

Greek-key motif
NMR structure
Northeast Structural Genomics Consortium
Ribosomal protein

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10.1110/ps.03359003

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@article{7987ce6950ac41d8b72dad31dc641824,

title = "Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii",

abstract = "We report NMR assignments and solution structure of the 71-residue 30S ribosomal protein S28E from the archaean Pyrococcus horikoshii, target JR19 of the Northeast Structural Genomics Consortium. The structure, determined rapidly with the aid of automated backbone resonance assignment (AutoAssign) and automated structure determination (AutoStructure) software, is characterized by a four-stranded β-sheet with a classic Greek-key topology and an oligonucleotide/oligosaccharide β-barrel (OB) fold. The electrostatic surface of S28E exhibits positive and negative patches on opposite sides, the former constituting a putative binding site for RNA. The 13 C-terminal residues of the protein contain a consensus sequence motif constituting the signature of the S28E protein family. Surprisingly, this C-terminal segment is unstructured in solution.",

keywords = "Greek-key motif, NMR structure, Northeast Structural Genomics Consortium, Ribosomal protein",

author = "Aramini, {James M.} and Huang, {Yuanpeng J.} and Cort, {John R.} and Sharon Goldsmith-Fischman and Rong Xiao and Shih, {Liang Yu} and Ho, {Chi K.} and Jinfeng Liu and Burkhard Rost and Barry Honig and Kennedy, {Michael A.} and Acton, {Thomas B.} and Montelione, {Gaetano T.}",

year = "2003",

month = dec,

doi = "10.1110/ps.03359003",

language = "English",

volume = "12",

pages = "2823--2830",

journal = "Protein Science",

issn = "0961-8368",

publisher = "Wiley-Blackwell",

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TY - JOUR

T1 - Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii

AU - Aramini, James M.

AU - Huang, Yuanpeng J.

AU - Cort, John R.

AU - Goldsmith-Fischman, Sharon

AU - Xiao, Rong

AU - Shih, Liang Yu

AU - Ho, Chi K.

AU - Liu, Jinfeng

AU - Rost, Burkhard

AU - Honig, Barry

AU - Kennedy, Michael A.

AU - Acton, Thomas B.

AU - Montelione, Gaetano T.

PY - 2003/12

Y1 - 2003/12

N2 - We report NMR assignments and solution structure of the 71-residue 30S ribosomal protein S28E from the archaean Pyrococcus horikoshii, target JR19 of the Northeast Structural Genomics Consortium. The structure, determined rapidly with the aid of automated backbone resonance assignment (AutoAssign) and automated structure determination (AutoStructure) software, is characterized by a four-stranded β-sheet with a classic Greek-key topology and an oligonucleotide/oligosaccharide β-barrel (OB) fold. The electrostatic surface of S28E exhibits positive and negative patches on opposite sides, the former constituting a putative binding site for RNA. The 13 C-terminal residues of the protein contain a consensus sequence motif constituting the signature of the S28E protein family. Surprisingly, this C-terminal segment is unstructured in solution.

AB - We report NMR assignments and solution structure of the 71-residue 30S ribosomal protein S28E from the archaean Pyrococcus horikoshii, target JR19 of the Northeast Structural Genomics Consortium. The structure, determined rapidly with the aid of automated backbone resonance assignment (AutoAssign) and automated structure determination (AutoStructure) software, is characterized by a four-stranded β-sheet with a classic Greek-key topology and an oligonucleotide/oligosaccharide β-barrel (OB) fold. The electrostatic surface of S28E exhibits positive and negative patches on opposite sides, the former constituting a putative binding site for RNA. The 13 C-terminal residues of the protein contain a consensus sequence motif constituting the signature of the S28E protein family. Surprisingly, this C-terminal segment is unstructured in solution.

KW - Greek-key motif

KW - NMR structure

KW - Northeast Structural Genomics Consortium

KW - Ribosomal protein

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DO - 10.1110/ps.03359003

M3 - Article

C2 - 14627742

AN - SCOPUS:10744222677

SN - 0961-8368

VL - 12

SP - 2823

EP - 2830

JO - Protein Science

JF - Protein Science

IS - 12

ER -

Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii

Abstract

Keywords

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