Softening of the packing density of horseradish peroxidase by a H-donor bound near the heme pocket

J. Fidy, J. M. Vanderkooi, J. Zollfrank, J. Friedrich

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23 Scopus citations

Abstract

We use pressure tuning of spectral holes to estimate the compressibility of protein molecules by optical means. We found that the compressibility of mesoporphyrin-substituted horseradish peroxidase increases by a factor of three when it incorporates small aromatic H-donor molecules that bind in the vicinity of its heme pocket. Such a dramatic softening of its packing density corresponds to a jump from a compressibility range characteristic for the solid state into that characteristic for liquids.

Original languageEnglish
Pages (from-to)1605-1612
Number of pages8
JournalBiophysical Journal
Volume63
Issue number6
DOIs
StatePublished - 1992
Externally publishedYes

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