Site selective and efficient spin labeling of proteins with a maleimide-functionalized trityl radical for pulsed dipolar EPR spectroscopy

Keyphrases

• Labeling Efficiency 100%
• Electron Paramagnetic Resonance Spectroscopy 100%
• Site-selective 100%
• Spin Label 100%
• Spin Labeling 100%
• Triarylmethyl Radical 100%
• Maleimide 100%
• Room Temperature 50%
• Cysteine 50%
• Cysteine Residues 50%
• Site-directed Spin Labeling 50%
• Electron Paramagnetic Resonance 50%
• Structural Biology 50%
• Resonance Experiment 50%
• Oligonucleotides 50%
• Yersinia Outer Protein 50%
• Methanethiosulfonate 50%
• Label Aggregation 50%
• Gem-dimethyl 50%
• Nitroxide Spin Label 50%

Biochemistry, Genetics and Molecular Biology

• Spin Labeling 100%
• Cysteine 100%
• Oligonucleotide 50%
• Site-Directed Spin Labeling 50%
• Yersinia 50%
• Room Temperature 50%

Chemistry

• EPR Spectroscopy 100%
• Maleimide 100%
• Cysteine 66%
• Spin Label 66%
• Ambient Reaction Temperature 33%
• Oligonucleotide 33%