SiRNA liposome-gold nanorod vectors for multispectral optoacoustic tomography theranostics

Adrian Taruttis; Neus Lozano; Antonio Nunes; Dhifaf A. Jasim; Nicolas Beziere; Eva Herzog; Kostas Kostarelos; Vasilis Ntziachristos

doi:10.1039/c4nr04164j

SiRNA liposome-gold nanorod vectors for multispectral optoacoustic tomography theranostics

Adrian Taruttis, Neus Lozano, Antonio Nunes, Dhifaf A. Jasim, Nicolas Beziere, Eva Herzog, Kostas Kostarelos, Vasilis Ntziachristos

Chair of Biological Imaging

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Therapeutic applications of gene silencing using siRNA have seen increasing interest over the past decade. The optimization of the delivery and biodistribution of siRNA using liposome-gold nanorod (AuNRs) nanoscale carriers can greatly benefit from adept imaging methods that can visualize the time-resolved delivery performance of such vectors. In this work, we describe the effect of AuNR length incorporated with liposomes and show their complexation with siRNA as a novel gene delivery vehicle. We demonstrate the application of multispectral optoacoustic tomography (MSOT) to longitudinally visualize the localisation of siRNA carrying liposome-AuNR hybrids within tumors. Combination of in vivo MSOT with ex vivo fluorescence cryo-slice imaging offers further insight into the siRNA transport and activity obtained.

Original language	English
Pages (from-to)	13451-13456
Number of pages	6
Journal	Nanoscale
Volume	6
Issue number	22
DOIs	https://doi.org/10.1039/c4nr04164j
State	Published - 21 Nov 2014

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abstract = "Therapeutic applications of gene silencing using siRNA have seen increasing interest over the past decade. The optimization of the delivery and biodistribution of siRNA using liposome-gold nanorod (AuNRs) nanoscale carriers can greatly benefit from adept imaging methods that can visualize the time-resolved delivery performance of such vectors. In this work, we describe the effect of AuNR length incorporated with liposomes and show their complexation with siRNA as a novel gene delivery vehicle. We demonstrate the application of multispectral optoacoustic tomography (MSOT) to longitudinally visualize the localisation of siRNA carrying liposome-AuNR hybrids within tumors. Combination of in vivo MSOT with ex vivo fluorescence cryo-slice imaging offers further insight into the siRNA transport and activity obtained.",

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AU - Taruttis, Adrian

AU - Lozano, Neus

AU - Nunes, Antonio

AU - Jasim, Dhifaf A.

AU - Beziere, Nicolas

AU - Herzog, Eva

AU - Kostarelos, Kostas

AU - Ntziachristos, Vasilis

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SiRNA liposome-gold nanorod vectors for multispectral optoacoustic tomography theranostics

Abstract

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