Sinorhizobium meliloti strain 1021 bioS and bdhA gene transcriptions are both affected by biotin available in defined medium

Kai Hofmann, Elke B. Heinz, Trevor C. Charles, Michael Hoppert, Wolfgang Liebl, Wolfgang R. Streit

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Sinorhizobium meliloti 1021 responds to external biotin signals from alfalfa plants through the bioS regulatory locus. Immunogold labeling and electron microscopy revealed that the BioS protein is located within the S. meliloti cytoplasm. Under biotin-limiting conditions the S. meliloti cell lumen was filled with polyhydroxybutyrate (PHB) granules suggesting that either PHB synthesis or degradation are influenced by biotin. To test this hypothesis a 3-hydroxybutyrate-dehydrogenase-lacZ (bdhA-lacZ) fusion was mobilized into S. meliloti. β-galactosidase tests revealed an overall 3.6-5.2-fold higher bdhA transcription in the presence of added biotin. Comparison of the bdhA and the bioS promoter regions identified several common motifs. Copyright (C) 2000 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)41-44
Number of pages4
JournalFEMS Microbiology Letters
Volume182
Issue number1
DOIs
StatePublished - 1 Jan 2000
Externally publishedYes

Keywords

  • Alfalfa
  • Biotin
  • Polyhydroxybutyrate
  • Rhizosphere
  • Root colonization
  • Sinorhizobium meliloti

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