Single-strand conformation polymorphism analysis of the epidermal growth factor receptor at codon 497

Martha E. Lopez; Michael S. Kobrin; Tetsuya Moriai; Munehiro Yokoyama; Helmut Friess; Markus Büchler; Murray Korc

doi:10.1097/00006676-199604000-00002

Single-strand conformation polymorphism analysis of the epidermal growth factor receptor at codon 497

Martha E. Lopez, Michael S. Kobrin, Tetsuya Moriai, Munehiro Yokoyama, Helmut Friess, Markus Büchler, Murray Korc

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A variant human epidermal growth factor (EGF) receptor (HER) with a transition (G to A) at codon 497, resulting in a substitution of a lysine for an arginine, was recently demonstrated in several human pancreatic cancer cell lines. In the present study, we compared the frequency of expression of wild-type (HER497R) and variant (HER497K) EGF receptors in normal and malignant pancreatic tissue samples using single-strand conformation polymorphism analysis. Both receptors were expressed in normal (42%) and malignant (46%) tissues. Three samples (two normal and one cancer) expressed only HER497K. The cancer sample that was homozygous for HER497K exhibited strong EGF receptor immunoreactivity. The high frequency of HER497K expression suggests that it is due to a relatively common polymorphism in the HER coding region. Its presence in some of the cancer samples suggests that, like the wild-type receptor, HER497K also has a role in pancreatic cancer.

Original language	English
Pages (from-to)	216-220
Number of pages	5
Journal	Pancreas
Volume	12
Issue number	3
DOIs	https://doi.org/10.1097/00006676-199604000-00002
State	Published - 1996
Externally published	Yes

Keywords

Epidermal growth factor receptor
Pancreas
Single-strand conformation polymorphism analysis
Variant

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1097/00006676-199604000-00002

Cite this

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title = "Single-strand conformation polymorphism analysis of the epidermal growth factor receptor at codon 497",

abstract = "A variant human epidermal growth factor (EGF) receptor (HER) with a transition (G to A) at codon 497, resulting in a substitution of a lysine for an arginine, was recently demonstrated in several human pancreatic cancer cell lines. In the present study, we compared the frequency of expression of wild-type (HER497R) and variant (HER497K) EGF receptors in normal and malignant pancreatic tissue samples using single-strand conformation polymorphism analysis. Both receptors were expressed in normal (42%) and malignant (46%) tissues. Three samples (two normal and one cancer) expressed only HER497K. The cancer sample that was homozygous for HER497K exhibited strong EGF receptor immunoreactivity. The high frequency of HER497K expression suggests that it is due to a relatively common polymorphism in the HER coding region. Its presence in some of the cancer samples suggests that, like the wild-type receptor, HER497K also has a role in pancreatic cancer.",

keywords = "Epidermal growth factor receptor, Pancreas, Single-strand conformation polymorphism analysis, Variant",

author = "Lopez, {Martha E.} and Kobrin, {Michael S.} and Tetsuya Moriai and Munehiro Yokoyama and Helmut Friess and Markus B{\"u}chler and Murray Korc",

year = "1996",

doi = "10.1097/00006676-199604000-00002",

language = "English",

volume = "12",

pages = "216--220",

journal = "Pancreas",

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publisher = "Lippincott Williams and Wilkins",

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T1 - Single-strand conformation polymorphism analysis of the epidermal growth factor receptor at codon 497

AU - Lopez, Martha E.

AU - Kobrin, Michael S.

AU - Moriai, Tetsuya

AU - Yokoyama, Munehiro

AU - Friess, Helmut

AU - Büchler, Markus

AU - Korc, Murray

PY - 1996

Y1 - 1996

N2 - A variant human epidermal growth factor (EGF) receptor (HER) with a transition (G to A) at codon 497, resulting in a substitution of a lysine for an arginine, was recently demonstrated in several human pancreatic cancer cell lines. In the present study, we compared the frequency of expression of wild-type (HER497R) and variant (HER497K) EGF receptors in normal and malignant pancreatic tissue samples using single-strand conformation polymorphism analysis. Both receptors were expressed in normal (42%) and malignant (46%) tissues. Three samples (two normal and one cancer) expressed only HER497K. The cancer sample that was homozygous for HER497K exhibited strong EGF receptor immunoreactivity. The high frequency of HER497K expression suggests that it is due to a relatively common polymorphism in the HER coding region. Its presence in some of the cancer samples suggests that, like the wild-type receptor, HER497K also has a role in pancreatic cancer.

AB - A variant human epidermal growth factor (EGF) receptor (HER) with a transition (G to A) at codon 497, resulting in a substitution of a lysine for an arginine, was recently demonstrated in several human pancreatic cancer cell lines. In the present study, we compared the frequency of expression of wild-type (HER497R) and variant (HER497K) EGF receptors in normal and malignant pancreatic tissue samples using single-strand conformation polymorphism analysis. Both receptors were expressed in normal (42%) and malignant (46%) tissues. Three samples (two normal and one cancer) expressed only HER497K. The cancer sample that was homozygous for HER497K exhibited strong EGF receptor immunoreactivity. The high frequency of HER497K expression suggests that it is due to a relatively common polymorphism in the HER coding region. Its presence in some of the cancer samples suggests that, like the wild-type receptor, HER497K also has a role in pancreatic cancer.

KW - Epidermal growth factor receptor

KW - Pancreas

KW - Single-strand conformation polymorphism analysis

KW - Variant

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U2 - 10.1097/00006676-199604000-00002

DO - 10.1097/00006676-199604000-00002

M3 - Article

C2 - 8830326

AN - SCOPUS:0029968858

SN - 0885-3177

VL - 12

SP - 216

EP - 220

JO - Pancreas

JF - Pancreas

IS - 3

ER -

Single-strand conformation polymorphism analysis of the epidermal growth factor receptor at codon 497

Abstract

Keywords

UN SDGs

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