Single-pair FRET characterization of DNA tweezers

Barbara K. Muller; Andreas Reuter; Friedrich C. Simmel; Don C. Lamb

doi:10.1021/nl0619406

Single-pair FRET characterization of DNA tweezers

Barbara K. Muller, Andreas Reuter, Friedrich C. Simmel, Don C. Lamb

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

The self-assembly properties of DNA make it an ideal choice for the construction of nanomachines such as DNA tweezers. Because nanomachines function as individual units, they need to be studied on the single-molecule level. From single-pair FRET investigations, we show that "open" tweezers exist in a single conformation with minimal FRET efficiency, whereas upon addition of a "closing strand", three conformations are observed, which are averaged out in ensemble measurements.

Original language	English
Pages (from-to)	2814-2820
Number of pages	7
Journal	Nano Letters
Volume	6
Issue number	12
DOIs	https://doi.org/10.1021/nl0619406
State	Published - Dec 2006
Externally published	Yes

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10.1021/nl0619406

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AB - The self-assembly properties of DNA make it an ideal choice for the construction of nanomachines such as DNA tweezers. Because nanomachines function as individual units, they need to be studied on the single-molecule level. From single-pair FRET investigations, we show that "open" tweezers exist in a single conformation with minimal FRET efficiency, whereas upon addition of a "closing strand", three conformations are observed, which are averaged out in ensemble measurements.

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Single-pair FRET characterization of DNA tweezers

Abstract

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