Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection

Annika Schneider, Sandra Kurz, Katrin Manske, Marianne Janas, Mathias Heikenwälder, Thomas Misgeld, Michaela Aichler, Sebastian Felix Weissmann, Hans Zischka, Percy Knolle, Dirk Wohlleber

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during viral infection of cells. Thus, single-organelle and multi-parameter resolution allows to explore altered energy metabolism and antiviral defence by tagged mitochondria selectively in virus-infected cells and will be instrumental to identify viral immune escape and to develop and monitor novel mitochondrial-targeted therapies.

Original languageEnglish
Article number8492
JournalScientific Reports
Volume9
Issue number1
DOIs
StatePublished - 1 Dec 2019

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