Simultaneous determination of amounts of major phospholipid classes and their fatty acid composition in erythrocyte membranes using high-performance liquid chromatography and gas chromatography

K. Eder, A. M. Reichlmayr-Lais, M. Kirchgessner

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

A method for the simultaneous determination of amounts of major phospholipid classes and their fatty acid composition in erythrocyte membranes is described. The method consists in extraction of phospholipids from erythrocyte membranes, separation of phospholipid classes by high-performance liquid chromatography, methylation of phospholipids and determination of phospholipid-bound fatty acids by capillary gas chromatography. The amounts of phospholipid classes are calculated from the total weight of phospholipid-bound fatty acids and their average molecular weights. The method was applied to erythrocytes from rats. The results show that the method is reproducible and is useful for the determination of amounts of phospholipid classes and their fatty acid composition in small blood samples.

Original languageEnglish
Pages (from-to)33-42
Number of pages10
JournalJournal of Chromatography A
Volume598
Issue number1
DOIs
StatePublished - 1 May 1992

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